Naslov
Metallothioneins and tissue metals in chronic relapsing form of experimental autoimmune encephalomyelytis in rats

Autori
Grebić, Damir ; Jakovac, Hrvoje ; Tota, Marin ; Barac-Latas, Vesna ; Mrakovčić-Šutić, Ines ; Milin, Čedomila ; Radošević-Stašić, Biserka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
European Journal of Immunology Vol. 39 Supplement 1 / Abstracts / - , 2009, S 493-S 493

Skup
2nd European Congress of Immunology ECI 2009

Mjesto i datum
Berlin, Njemačka, 13.09.2009. - 16.09.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
metallothioneins I+II; tissue metals; experimental autoimmune encephalomyelitis; DA rats;

Sažetak
Aim: In the genetically susceptible Dark Agouti rats (DA) experimental autoimmune encephalomyelitis may be induced in chronic relapsing form (CR-EAE) that resembles to multiple sclerosis, which is an inflammatory, demyelinating disease the central nervous system. It is caused by erroneous activation of self-reactive T cells specific for myelin antigen, but the severity of symptoms depends on the balance between the aggressive and the localized, or systemic protective tissue reaction to infection or injury. Methods: Since in the pathogenesis of disease a significant role may have the metallothioneins (MTs), which are the antioxidant proteins with high metal-binding properties, in this study we estimated the expression of MTs I+II, as well as the tissue concentrations of Zn2+ and Cu2+ in the brain, spinal cord and liver during the first and second attack (on the 12th and 22nd post-immunization day, respectively) and during the remission phases (on the 18th and 28th day) of CR-EAE, induced in DA rats by subcutaneous injection of encephalitogen (bovine brain homogenate in complete Freund’ s adjuvant). Controls consisted of rats treated only with CFA. Clinical assessment was performed according to the standard criteria, while MTs and metal ions were analyzed by immunocytochemistry and inductivity coupled plasma spectrometry, respectively. Results: The data showed that during the first attack MTs I+II were upregulated in the brain (subpial region, perivascular space and parenchymal astrocytes) and in the spinal cord (glial cells and neurons). Simultaneously, increased the concentration of Zn2+ in the spinal cord and Zn2+ and Cu2+ in the liver. During the second attack a very high, new overexpression was found in the molecular layer of cerebellum, in sulcus hippocampi, on several neurons and oligodendrocytes in spinal corn, and particularly on hepatocytes around the central vein. Simultaneously, in the brain and spinal cord increased the concentration of Cu2+. Conclusions: The data point to neuroprotective role of MTs and to important regulatory role of essential metals and hepatic MTs for the recovery from acute attacks of EAE.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA