Naslov
Solid Phase Extraction of Polyphenolics from Propolis

Autori
Medić-Šarić, Marica ; Pucl, Matijana ; Bojić, Mirza

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
8th Balaton Symposium on High Performance Separation Methods and 15th International Symposium on Separation Sciences: Book of Abstracts / Felinger, Attila ; Felinger, Gabriella - Pečuh : Hungarian Society for Separation Sciences, 2009, 196-196

ISBN
978-963-06-7878-0

Skup
8th Balaton Symposium on High Performance Separation Methods and 15th International Symposium on Separation Sciences

Mjesto i datum
Siófok, Mađarska, 02.09.2009. - 04.09.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
SPE; Propolis

Sažetak
Propolis is a complex mixture of which polyphenolics, flavonoids and phenolic acids are the most important for the pharmacological activity (e.g. antioxidative, antiimmflamatory, immunomodulatory, antimicrobial). To analyse such a mixture there is necessity for purification and concentration, both of which can easily be achieved by solid phase extraction. As phenolic acids and flavonoids present weak acids we used mixed-mode anion-exchange and reversed-phase sorbent syringe cartridges (Oasis&reg ; ; MAX) for separation. Separation was done on the mixture of standards (phenolic acids: ferulic, caffeic, isoferulic, p-coumaric ; flavonoids: apigenin, chrysin, naringenin, galangin, quercetin, pinocembrin, kaempferol, pinostrobin, rhamnetin, sakuranetin and tectochrysin) and the mixture of propolis (samples from Pelješac, Vis, Metković, Omiš, Sisak, Sinj and Veliki Zdenci) using binary gradient method of 2% acetic acid in methanol and 2% acetic acid in water modified with 10% of water and methanol, respectively. Collected fractions were analysed using high performance liquid chromatography with DAD detector. The complete separation of standards of phenolic acids from flavonoids was possible, phenolic acids being the eluted first. Recovery rate of flavonoids was rather high (94%) compared to phenolic acids (66%). Clear separation of polyphenolcis from propolis using binary gradient method was not achieved only for naringenin being the most polar flavonoid from standards used.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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