Naslov
Toxic effects of metabolites of indoor moulds

Autori
Pieckova, E ; Kollarikova, Z ; Kovačikova, Z ; Hurbankova, M ; Černa, S ; Liškova, A ; Tatrai, E ; Šegvić Klarić, Maja ; Bloom, E

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
6th Pan African Environmental Mutagen Society Conference (PAEMS 2008) / - , 2008, 107-107

Skup
6th Pan African Environmental Mutagen Society Conference (PAEMS 2008)

Mjesto i datum
Cape Town, Južnoafrička Republika, 02.11.2008. - 05.11.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
A. veriscolor; Stachybotrys; sterigmatocystin; patulin; ochratoxin A

Sažetak
There are many reports of allergies, chronic intoxication or even lung hemosiderosis of workers and occupants in buildings contaminated with indoor moulds. Mechanisms of the potential pathological effects of fungal toxic compounds on airways of healthy humans have not been sufficiently clariffied yet. In vitro and in vivo toxicity of the most frequent indoor mycromycetes- under Slovak dwellings conditions (Aspergillus veriscolor, A. ustus, Penicillium expansum, P. Chrysogenum) and top risky Stachybotrys chartarum-from the public health point of view-with extrolites profile characterised by TLC and LC/MS/MS-was studied by microbiological methods (employing Bacillus subtilis), ba a bioassay on chick tracheal cultures and by analyses of histological and biochemical alterations of rat organs or cell cultures and cytotoxic, infalmmatory and hematological parameters in bronchoalveolar lavage fluid or blood. Pure solvent (2% dimethylsulfoxide-negative control) and tandards of mycotoxins, all carcinogenic, potentially produced by the fungus tested (sterigmatocystin, patulin, ochratoxin A, trichotecenes) were used-20 ug/mL (in vitro studies) and 4 ug/mL (in vivo, intratracheal instilation), exposure for 3 days. All fungal metabolites tested showed certain toxic effects (bacterial growth inhibition, caesing tracheal epithelial cells beating, statistically significant changes in hematological parameters and lung injury-cytotoxic and inflammatory-detectable by bronchoalveolar lavage) that were concetration and cell origin of the toxicant (exo- or endocellular metabolite) dependent. Generall, exometabolites were able to damage natural cell and organ functions of airways in vitro/in vivo much stronger.

Izvorni jezik
Engleski

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