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Pregled bibliografske jedinice broj: 425086

Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction

Penčik, Aleš; Rolčik, Jakub; Novak, Ondrej; Magnus, Volker; Bartak, Petr; Buchtik, Roman; Salopek-Sondi, Branka; Strnad, Miroslav
Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction // Talanta, 80 (2009), 2; 651-655 doi:10.1016/j.talanta.2009.07.043 (međunarodna recenzija, članak, znanstveni)

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Naslov
Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction

Autori
Penčik, Aleš ; Rolčik, Jakub ; Novak, Ondrej ; Magnus, Volker ; Bartak, Petr ; Buchtik, Roman ; Salopek-Sondi, Branka ; Strnad, Miroslav

Izvornik
Talanta (0039-9140) 80 (2009), 2; 651-655

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Immunoaffinity extraction; High-performance liquide chromatography (HPLC); Tandem mass spectrometry (MS/MS); Indole-3-acetic acid (IAA); Auxin conjugates; Helleborus niger L.

Sažetak
An analytical protocol for the isolation and quantification of indole-3-acetic acid (IAA) and its amino acid conjugates was developed. IAA is an important phytohormone and formation of its conjugates plays a crucial role in regulating IAA levels in plants. The developed protocol combines a high-specific immunoaffinity extraction with a sensitive and selective LC-MS/MS analysis. By using internal standards for each of the studied compouns, IAA and seven amino acid conjugates were analyzed in quantitie of fresh plant material as low as 30 mg. In seed of Helleborus niger, physiological levels of these compounds were found to range from 7.5 nmolg-1 fresh weight (IAA) to 0.44 pmolg-1 fresh weight (conjugate with Ala). To our knowledge, the identification of IAA conjugates with Gly, Phe, and Val from higher plants is reported here for the first time.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA

Projekti:
098-0982913-2829 - Molekularna regulacija biljnog razvitka (Salopek-Sondi, Branka, MZOS ) ( CroRIS)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Volker Magnus (autor)

Avatar Url Branka Salopek-Sondi (autor)

Poveznice na cjeloviti tekst rada:

Pristup cjelovitom tekstu rada doi www.sciencedirect.com

Citiraj ovu publikaciju:

Penčik, Aleš; Rolčik, Jakub; Novak, Ondrej; Magnus, Volker; Bartak, Petr; Buchtik, Roman; Salopek-Sondi, Branka; Strnad, Miroslav
Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction // Talanta, 80 (2009), 2; 651-655 doi:10.1016/j.talanta.2009.07.043 (međunarodna recenzija, članak, znanstveni)
Penčik, A., Rolčik, J., Novak, O., Magnus, V., Bartak, P., Buchtik, R., Salopek-Sondi, B. & Strnad, M. (2009) Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction. Talanta, 80 (2), 651-655 doi:10.1016/j.talanta.2009.07.043.
@article{article, author = {Pen\v{c}ik, Ale\v{s} and Rol\v{c}ik, Jakub and Novak, Ondrej and Magnus, Volker and Bartak, Petr and Buchtik, Roman and Salopek-Sondi, Branka and Strnad, Miroslav}, year = {2009}, pages = {651-655}, DOI = {10.1016/j.talanta.2009.07.043}, keywords = {Immunoaffinity extraction, High-performance liquide chromatography (HPLC), Tandem mass spectrometry (MS/MS), Indole-3-acetic acid (IAA), Auxin conjugates, Helleborus niger L.}, journal = {Talanta}, doi = {10.1016/j.talanta.2009.07.043}, volume = {80}, number = {2}, issn = {0039-9140}, title = {Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction}, keyword = {Immunoaffinity extraction, High-performance liquide chromatography (HPLC), Tandem mass spectrometry (MS/MS), Indole-3-acetic acid (IAA), Auxin conjugates, Helleborus niger L.} }
@article{article, author = {Pen\v{c}ik, Ale\v{s} and Rol\v{c}ik, Jakub and Novak, Ondrej and Magnus, Volker and Bartak, Petr and Buchtik, Roman and Salopek-Sondi, Branka and Strnad, Miroslav}, year = {2009}, pages = {651-655}, DOI = {10.1016/j.talanta.2009.07.043}, keywords = {Immunoaffinity extraction, High-performance liquide chromatography (HPLC), Tandem mass spectrometry (MS/MS), Indole-3-acetic acid (IAA), Auxin conjugates, Helleborus niger L.}, journal = {Talanta}, doi = {10.1016/j.talanta.2009.07.043}, volume = {80}, number = {2}, issn = {0039-9140}, title = {Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction}, keyword = {Immunoaffinity extraction, High-performance liquide chromatography (HPLC), Tandem mass spectrometry (MS/MS), Indole-3-acetic acid (IAA), Auxin conjugates, Helleborus niger L.} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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