Naslov
IS1245-RFLP based genetic relatedness of the Mycobacterium avium subsp. hominissuis strains isolated from humans, animals and environment in Croatia

Autori
Špičić, Silvio ; Cvetnić, Željko ; Pate, Mateja ; Katalinić-Janković, Vera ; Duvnjak, Sanja ; Ocepek, Matjaž ; Zdelar-Tuk, Maja ; Krt, Brane

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
ESM 30th Annual Congress, Porto, Portugal / Suzana David - Porto, 2009

Skup
European Society of Mycobacteriology 30th Annual Congress

Mjesto i datum
Porto, Portugal, 05.07.2009. - 08.07.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Mycobacterium avium subsp. hominissuis; RFLP

Sažetak
Significance of infections with Mycobacterium avium, in particular subspecies M. a. hominissuis, in animals and humans is constantly increasing. Susceptibility of humans, cattle and swine to various mycobacteria and the prevalence of these bacteria in the environment, although very important from the aspect of epizootiology and epidemiology, have not been sufficiently investigated in Croatia. This study is based on massive tuberculin skin tests of cattle and swine from large farms, bacteriology and molecular identification of M. a.hominissuis isolated from domestic and wild animals, humans and environment. Genotyping of the 23 isolates was conducted by IS1245 RFLP method. The selected cut-off value for cluster designation was similarity level of 75%. A total of 5 clusters containing 86.9 % of all genotyped strains were identified. High similarity level of the profiles within a cluster was found for the isolates from man, swine from intensive breeding, deer, wild boar and sawdust from various counties. As no epizootiological links between animals and humans were identified, humans and animals could be considered as dead-end hosts. According to our results, the source of infection for humans and animals is most probably the environment.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita, Veterinarska medicina

POVEZANOST RADA