Naslov
Hsp70, TNFalpha and hsp32 gene polymorphisms and COPD

Autori
Matokanović, Mirela ; Malić, Ana ; Turk, Ana ; Čepelak, Ivana ; Popović-Grle, Sanja ; Barišić, Karmela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
The FEBS Journal. 276, Supplement 1 (2009), Abstracts of the 34th FEBS Congress / - : Wiley-Blackwell, 2009, 327-327

Skup
34th FEBS Congress, Life's Molecular Interactions

Mjesto i datum
Prag, Češka Republika, 04.07.2009. - 09.07.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Hsp70-2; hsp70-hom; TNFα; hsp32; polymorphism; COPD

Sažetak
Genes encoding members of the heat shock protein (Hsp) 70 family, hsp70-1, hsp70-2 and hsp-hom, and gene encoding tumor necrosis factor alpha (TNFα ) lie in the class III region of the human major histocompatibility complex (MHC). Hsp70-1 and hsp70-2 genes encode the major heat and stress inducible Hsp70. Hsp70-hom encodes a protein that is highly related to Hsp70-1, but is not heat inducible. TNFα encodes pro-inflammatory cytokine, which plays an important role in inflammatory processes. Hsp32 gene (HOX-1) encodes inducible heme oxygenase-1, enzyme that catalyzes the degradation of heme, providing cellular protection against oxidative mediated injury. Polymorphisms of stress (hsp70 family and hsp32) and tumor necrosis factor alpha (TNFα ) genes have been reported to be related to chronic obstructive pulmonary disease (COPD), a disease associated with an immune as well as an oxidant/anti-oxidant imbalance. Therefore hsp70-2 A/G (+ 1267), hsp70-hom T/C (+ 2437) and TNFα G/A (+489) gene polymorphisms were analysed in patients with COPD by PCR-RFLP procedure. Polymorphisms of the HOX-1 gene regarding to the number of GT repeats in 5’ -flanking region were analysed with sequencing of PCR products. The obtained data showed that there were no statistically significant differences in the genotype distributions of hsp70-2 (Chi square test, P = 0, 186), hsp70-hom (Chi square test, P = 0, 859) and TNFα (Chi square test, P = 0, 299) between patients (31) with COPD and control subjects (48). Likewise there was no statistically significant difference in number of GT repeats in HOX-1 gene between patients with COPD and control subjects (Chi square test, P = 0, 907).

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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