Pregled bibliografske jedinice broj: 4218
Modulation of apurinic (AP) endonuclease activity in mammalian cells: transfection of yeast, but not human AP endonuclease renders cells more resistant to genotoxic agents
Modulation of apurinic (AP) endonuclease activity in mammalian cells: transfection of yeast, but not human AP endonuclease renders cells more resistant to genotoxic agents // Archives of Pharmacology / Gothert, M. ; Jakobs, K. H. (ur.).
Berlin: Springer, 1997. (poster, nije recenziran, sažetak, znanstveni)
CROSBI ID: 4218 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Modulation of apurinic (AP) endonuclease activity in mammalian cells: transfection of yeast, but not human AP endonuclease renders cells more resistant to genotoxic agents
Autori
Tomičić, Maja ; Kaina, Bernd
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Archives of Pharmacology
/ Gothert, M. ; Jakobs, K. H. - Berlin : Springer, 1997
Skup
Deutsche Genellschaft fur experimentelle und klinische Pharmakologie und Toxikologie
Mjesto i datum
Mainz, Njemačka, 11.03.1997. - 13.03.1997
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
stanice sisavaca; apurinska endonuklearna aktivnost; genotoksični agensi
(mammalian cells; apurinic endonuclease activity; genotoxic agents)
Sažetak
Abasic sites are ubiquitous DNA lesions that arise spontaneously or are induced by DNA damaging agents. They block DNA replication and are cytotoxic and mutagenic. Key enzymes involved in repair of abasic sites are apurinic/apyrimidinic endonucleases (APendo). To analyze the role of APendo in protection of mammalian cells against DNA damaging agents, we have transfected both the human (APE) and the yeast (APN1) AP endonuclease in Chinese hamster cells and compared the effects of expression of these genes in stable transfectants regarding survival of cells and chromosomal aberration formation. Although APE was markedly expressed on RNA and protein level, nuclear extracts of human APE transfectants did not show a higher APendo activity than the parental line and became not more resistant to the cell kiling and clastogenic effect of methyl methanesulfonate and hydrogen peroxide. In constrast, cells transfected with the yeast APN1 gene expressed higher APendo activity and became clearly more resistant to the cytotoxic and chromosome breakage inducing activity of the agents. The results indicate that the excision repair capacity and mutagen resistance can be enhanced by introducing, in mammalian cells, a yeast DNA repair gene. They also verify that AP sites are both cytotoxic and clastogenic lesions.
Izvorni jezik
Engleski
