Naslov
Genotyping of chlamydial samples isolated from birds in Croatia

Autori
Prukner-Radovcic, Estella ; Karine Laroucau ; Danijela Horvatek ; Željko Gottstein ; Fabien Vorimore

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings of the First European Meeting on Animal Chlamydioses and Zoonotic Aspects / Caro, Maria R. ; Salinas, Jesus ; Buendia, Antonio J. - Murcia : Centro Social Universitario Campus de Espinardo, 2009, 40-40

Skup
First European Meeting on Animal Chlamydioses and Zoonotic Aspects

Mjesto i datum
Murcia, Španjolska, 14.06.2009. - 16.06.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
chlamydia; genotyping; birds

Sažetak
Obligate intracellular bacterium Chlamydophila psittaci is causative agent of avian chlamydiosis and human psittacosis. In the Republic of Croatia, according to state laws, all avian flocks and birds in transport must be tested for avian chlamydiosis, especially because their zoonotic implication. As intracellular and zoonotic agent it represents difficult and hazardous material for handling and diagnostics. During last 3 years samples from different bird species (mostly fecal samples, swabs or organs) were examined by using several diagnostic methods ; however, since 2008, diagnostics is based on conventional PCR and real-time PCR. Those molecular methods are specific, sensitive and allow rapid detection without the need for culturing of Chlamydia (Borel et al., 2008). Ten positive samples obtained from birds with clinical signs were genotyped in French laboratory using specific real-time PCR and alternate molecular tools- C. psittaci specific multilocus variable number of tandem repeats (MLVA) scheme or restriction fragment length polymorphism (PCR-RFLP). As expected, most of the samples (altogether 7) belonged to the C. psittaci species. Genotyping by PCR-RFLP and MLVA revealed the presence of one serovar A (from a parrot) and serovar E/B from 6 pigeons. Genotypes of the samples by MLVA were determined when the DNA concentration allowed it. Surprisingly, 3 of PCR positive samples (1 sample isolated from a laying hen and 2 from turkeys) didn’ t react with any of the C. psittaci specific tool (real-time PCR, MLVA, ) as well as C. abortus and C. pecorum specific tools. As recently novel Chlamydophila spp. strains have been isolated from poultry (Laroucau et al., submitted) and as a specific real-time PCR has been developed, the analysis of these 3 odds samples showed relatedness to newly isolated Chlamydophila spp. Sequencing of the ompA and 16S gene of one of these samples confirmed this data, which suggested that novel Chlamydophila spp. are more widely spread than initially suspected. Cooperation between two laboratories resulted in first genotyping of Croatian chlamydial samples, and further investigations will help in monitoring, but also in prevention of chlamydiosis in birds and in humans. This investigation was partly supported by the ministry of Science and Technology of the Republic of Croatia, Grant No. 053-053-1863-1857

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA