Naslov
Lipid modifications by free radicals: Peroxidation vs. Isomerization

Autori
Tartaro, Ivana ; Mihaljević, Branka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
European Young Investigator Conference, Slubice, Poland, 2009 / - , 2009

Skup
4th European Young Investigator Conference 2009

Mjesto i datum
Słubice, Poljska, 18.06.2009. - 21.06.2009

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
gamma irradiation; lipid peroxidation; isomerization; micelle

Sažetak
During oxidative stress reactive oxygen species and peroxyl radicals of lipids can be scavenged by good hydrogen donors such as thiols, leading to interrupting the cascade of the lipid peroxidation process. Therefore thiols are considered to be effective protectors for the lipid degradation under oxidative conditions. On the other side, thiyl radicals generated from thiols by free radicals cause the isomerization of the double bonds, with the formation of geometrical lipid trans isomers.The general objective of this work was a comparative study of the behaviour of unsaturated fatty acid in the presence of thiols. Primary radicals were generated by γ -irradiation of a model lipid system containing linoleic acid (LiH), nonionic surfactant (Tween 20) and 2-mercaptoethanol at pH 5. After γ -irradiation of the model systems up to 400 Gy and in the presence of oxygen, high concentration of LiOOH (1 × 10– 4 mol dm-3) was determined, while the amount of trans LiH methyl ester simultaneously formed under the same conditions was very low (~ 10 %). However, in the absence of oxygen the effective inhibition of propagation of lipid peroxidation with subsequent increasing of the isomerization processes was observed. It was found that the amount of trans isomers increased with increasing of the irradiation dose. The effect of the dose rate on the lipid modification processes was also investigated.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA