Naslov
Comparison of different root canal sealers: cytotoxicity and the type of induced cell death

Autori
Brzović, Valentina ; Miletić, Ivana ; Anić, Ivica ; Vuković, Lidija ; Kqiku, Lumnije ; Osmak, Maja

Izvornik
Stomatologie (0946-3151) 106 (2009); 47-51

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Apoptosis ; cytotoxicity ; necrosis ; root canal

Sažetak
The aim of this study was to compare in vitro cytotoxicity and determine the type of cell death induced by Hermetic, Diaket, GuttaFlow, Epiphany, SuperEBA and IRM using human laryngeal carcinoma HEp2 cells as the model system. The cytotoxicity was determined by counting the total number of cells by electronic counter, while the number of viable cells was estimated under a light microscope. The number of apoptotic or necrotic cells was estimated under a fluorescent microscope according to their characteristic morphology and staining with specific fluorescent dyes. The results show that GuttaFlow was biocompatible, hardly reducing the number of living human laryngeal carcinoma HEp2 cells thorough all research periods(except after one month period). Different from that, Diaket, Epiphany, IRM, Hermetic, and Super EBA exhibited strong toxicity. Diaket and IRM mostly induced apoptosis while for Epiphany, Hermetic and Super EBA, necrosis was the predominant or only type of cell death.

Izvorni jezik
Engleski

Znanstvena područja
Dentalna medicina

POVEZANOST RADA