Naslov
Design of species specific primers for amplification of mitochondrial DNA of Croatian wild animals

Autori
Pađen, Lana ; Sadarić, Ivana ; Gomerčić, Tomislav ; Sindičić, Magda ; Đuras Gomerčić, Martina ; Slavica, Alen

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Proceedings of the International Scientific Meeting of Anatomy and Physiology Fundamentals of Medicine / Mihelić, Damir ; Šimpraga, Miljenko ; Tkalčić, Suzana - Zagreb, 2009, 129-137

ISBN
978-953-6062-72-0

Skup
International Scientific Meeting of Anatomy and Physiology Fundamentals of Medicine

Mjesto i datum
Zagreb, Hrvatska, 12.06.2009. - 13.06.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
primer; mitochondrial DNA; control region; wild animal

Sažetak
The goal of this paper was to design species specific primers for wolf (Canis lupus), fox (Vulpes vulpes), bear (Ursus arctos), roe deer (Capreolus capreolus), red deer (Cervus elaphus) and wild boar (Sus crofa). Primers have been designed using sequences of the listed species from the GenBank, using software BioEdit and Primer 3. We have defined the size and location of primers for each species, and size of the PCR product. With this species specific primers animal species can be identified using minimal quantities of different tissues using only DNA isolation, PCR and electrophoresis, so they can be used in a variety of ecological researches and in forensic cases.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA