Naslov
Development of anterior pituitary cell culture system for toxicological application

Autori
Zechner-Krpan, Vesna ; Kniewald, Jasna ; Kniewald, Zlatko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
8th European Congress on Biotechnology - Book of Abstracts / Laszlo, Nyeste - Budimpešta : Hungarian Biochemical Society, 1997

Skup
8th European Congress on Biotechnology - 70th Event of the European Federation of Biotechnology

Mjesto i datum
Budimpešta, Mađarska, 17.08.1997. - 21.08.1997

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
anterior pituitary; primary cell culture; hormone secretion

Sažetak
Anterior pituitary cells in culture for studing the hormone secretion provides a number of advantages in in vitro experiments. Primary culture of pituitary cells allow complete isolation of the pituitary from neural and humoral influence, and addition of drugs or xenobiotics including environmental pollutants under a controlled environment. For preparing primary cell culture from male rat anterior pituitary the mechanical disruption method and short-term enzymatic dispersion with collagenase were used. The optimal density of the cell suspension was between 1-5x105 cells/ml. The cells were plated in medium M-199 (pH 7.6) containing 10% fetal calf serum. Plated pituitary cells attached quickly (within 24 hrs) and quantitatively (85%) to plastic surfaces of plates or wells. Cell colonies were formed during a 3-day culture period as the cells flattened and re-established contacts with neighboring cells. Cultured pituitary cells permit examination of long-term effects of drugs or xenobiotics on pituitary hormone secretion in controlled conditions. Though pituitary cells after isolation from tissue and culturing showed comparable activity with tissue culture experiments made previously. It is advaisable before utilizing cultured cells in toxicological studies to investigate this secretory system by comparison with a well established in vitro model. J.Kniewald et al, J.appl.toxicol. 14 (1994) and 16 (1996).

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA