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Pregled bibliografske jedinice broj: 412935

Comparison of molecular assays for detection and typing of human papillomavirus

Koidl, Christoph; Božić, Michale; Hadžisejdić, Ita; Grahovac, Maja; Grahovac, Blaženka; Kranewitter, Wolfgang; Marth, Egon; Kessler, Harald H.
Comparison of molecular assays for detection and typing of human papillomavirus // American Journal of Obstetrics and Gynecology, 199 (2008), 2; E1-1 doi:10.1016/j.ajog.2008.03.005 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 412935 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Comparison of molecular assays for detection and typing of human papillomavirus

Autori
Koidl, Christoph ; Božić, Michale ; Hadžisejdić, Ita ; Grahovac, Maja ; Grahovac, Blaženka ; Kranewitter, Wolfgang ; Marth, Egon ; Kessler, Harald H.

Izvornik
American Journal of Obstetrics and Gynecology (0002-9378) 199 (2008), 2; E1-1

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA extraction; human papillomavirus; microarray;

Sažetak
The objective of the study was to compare the performance of 3 different extraction instruments in conjunction with 4 different amplification and detection kits for detection and typing of human papillomavirus (HPV) deoxyribonucleic acid (DNA). A total of 42 cervical swabs were investigated. HPV DNA was extracted on the 3 different instruments. Each of the extracts was then amplified, and HPV DNA amplification products were detected with 4 different kits. In 31 samples, HPV DNA was detected by both the Amplicor HPV test and the LINEAR ARRAY HPV genotyping test in conjunction with DNA extraction on the easyMAG instrument. In another 6 samples, only low-risk types were detected with the LINEAR ARRAY HPV genotyping test. After extraction on the easyMAG instrument, 32 samples tested positive when the PapilloCheck with the HotStarTaq DNA polymerase was used. Together with extraction on the easyMAG instrument, the Amplicor HPV test, the LINEAR ARRAY HPV genotyping test, and the new PapilloCheck with the HotStarTaq DNA polymerase provide comparable results allowing reliable and safe HPV diagnostics in the routine laboratory. Use of alternative assays may lead to an increase of invalid and divergent HPV typing results. Key words: DNA extraction, human papillomavirus, microarray, polymerase chain reaction, typing

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

Napomena
Art. no. 144.e1.



POVEZANOST RADA

Projekti:
062-0000000-3550 - Molekularni mehanizmi infekcije virusom hepatitisa C (Grahovac, Blaženka, MZOS ) ( CroRIS)

Ustanove:
Medicinski fakultet, Rijeka

Profili:

Avatar Url Blaženka Grahovac (autor)

Avatar Url Ita Hadžisejdić (autor)

Poveznice na cjeloviti tekst rada:

doi

Citiraj ovu publikaciju:

Koidl, Christoph; Božić, Michale; Hadžisejdić, Ita; Grahovac, Maja; Grahovac, Blaženka; Kranewitter, Wolfgang; Marth, Egon; Kessler, Harald H.
Comparison of molecular assays for detection and typing of human papillomavirus // American Journal of Obstetrics and Gynecology, 199 (2008), 2; E1-1 doi:10.1016/j.ajog.2008.03.005 (međunarodna recenzija, članak, znanstveni)
Koidl, C., Božić, M., Hadžisejdić, I., Grahovac, M., Grahovac, B., Kranewitter, W., Marth, E. & Kessler, H. (2008) Comparison of molecular assays for detection and typing of human papillomavirus. American Journal of Obstetrics and Gynecology, 199 (2), E1-1 doi:10.1016/j.ajog.2008.03.005.
@article{article, author = {Koidl, Christoph and Bo\v{z}i\'{c}, Michale and Had\v{z}isejdi\'{c}, Ita and Grahovac, Maja and Grahovac, Bla\v{z}enka and Kranewitter, Wolfgang and Marth, Egon and Kessler, Harald H.}, year = {2008}, pages = {E1-1-E1-6}, DOI = {10.1016/j.ajog.2008.03.005}, keywords = {DNA extraction, human papillomavirus, microarray, }, journal = {American Journal of Obstetrics and Gynecology}, doi = {10.1016/j.ajog.2008.03.005}, volume = {199}, number = {2}, issn = {0002-9378}, title = {Comparison of molecular assays for detection and typing of human papillomavirus}, keyword = {DNA extraction, human papillomavirus, microarray, } }
@article{article, author = {Koidl, Christoph and Bo\v{z}i\'{c}, Michale and Had\v{z}isejdi\'{c}, Ita and Grahovac, Maja and Grahovac, Bla\v{z}enka and Kranewitter, Wolfgang and Marth, Egon and Kessler, Harald H.}, year = {2008}, pages = {E1-1-E1-6}, DOI = {10.1016/j.ajog.2008.03.005}, keywords = {DNA extraction, human papillomavirus, microarray, }, journal = {American Journal of Obstetrics and Gynecology}, doi = {10.1016/j.ajog.2008.03.005}, volume = {199}, number = {2}, issn = {0002-9378}, title = {Comparison of molecular assays for detection and typing of human papillomavirus}, keyword = {DNA extraction, human papillomavirus, microarray, } }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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