Pregled bibliografske jedinice broj: 41204
Structural aspects of thiol-specific spin labeling of human plasma low density lipoprotein
Structural aspects of thiol-specific spin labeling of human plasma low density lipoprotein // Biopolymers, 57 (2000), 6; 336-343 doi:10.1002/1097-0282(2000)57:63.0.CO ; 2-1 (međunarodna recenzija, članak, znanstveni)
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Naslov
Structural aspects of thiol-specific spin labeling of human plasma low density lipoprotein
Autori
Kveder, Marina ; Pifat, Greta ; Vukelić, Bojana ; Pečar, Slavko ; Schara, Milan
Izvornik
Biopolymers (0006-3525) 57
(2000), 6;
336-343
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
low density lipoprotein ; electron paramagnetic resonance ; thiol spin labeling of apoprotein B
Sažetak
A novel thiol-specific spin labeling procedure for the protein component (apoprotein B, apoB) of low density lipoproteins (LDLs) is presented. A methanethiosulfonate spin label was used to probe the free cysteine residues of apoB with electron paramagnetic resonance (EPR) spectroscopy. The results indicated that the spin labeled sites are predominantly buried in the LDL particle in two distinct environments that differ in their mobility restrictions. The suitability of thiol-specific labeling for the study of the stability and conformation of apoB was demonstrated in experiments with denaturing agents. The results presented in this work offer a new approach for the matching of EPR data with the primary structure of apoB.
Izvorni jezik
Engleski
Znanstvena područja
Kemija
POVEZANOST RADA
Ustanove:
Institut "Ruđer Bošković", Zagreb
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
Uključenost u ostale bibliografske baze podataka::
- Biological Abstracts
- Chemical Abstracts
- Excerpta Medica
- Index Medicus
