Naslov
Identification of Differentially Expressed Genes Between Osteoblasts and Osteocytes

Autori
Paić, Frane ; Igwe, John C. ; Ravi, Nori ; Kronenberg, Mark S. ; Franceschetti, Tiziana ; Harrington, Patrick ; Kuo, Lynn ; Shin, Don-Guk ; Rowe, David W. ; Harris, Stephen E. ; Kalajzić, Ivo

Izvornik
Bone (8756-3282) 45 (2009), 4; 682-692

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Col2.3; Dentin matrix protein 1; osteoblast; osteocyte; microarray; GFP

Sažetak
Osteocytes represent the most abundant cellular component of mammalian bones with important functions in bone mass maintenance and remodeling. To elucidate the differential gene expression between osteoblasts and osteocytes we completed a comprehensive analysis of their gene profiles. Selective identification of these two mature populations was achieved by utilization of visual markers of bone lineage cells. We have utilized dual GFP reporter mice in which osteocytes are expressing GFP (topaz) directed by the DMP1 promoter, while osteoblasts are identified by expression of GFP (cyan) driven by 2.3kb of the Col1a1 promoter. Histological analysis of 7-day-old neonatal calvaria confirmed the expression pattern of DMP1GFP in osteocytes and Col2.3GFP in osteoblasts and osteocytes. To isolate distinct populations of cells we utilized fluorescent activated cell sorting (FACS). Cells suspensions were subjected to RNA extraction, in vitro transcription and labeling of cDNA and gene expression was analyzed using the Illumina WG-6v1 BeadChip.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA