Naslov
Histomorphometric characteristics of immune cells in small intestine of pigs perorally immunized with F18ac+ nonenterotoxigenic E. coli vaccinal strain

Autori
Kovšca Janjatović, Ana ; Lacković, Gordana ; Božić, Frane ; Špoljarić, Daniel ; Popović, Maja ; Valpotić, Hrvoje ; Vijtiuk, Nada ; Pavičić, Željko ; Valpotić, Ivica

Izvornik
European journal of histochemistry (1121-760X) 53 (2009), 4; 189-198

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
E. coli; gut immune cells; pigs; nonspecific/specific immunization;

Sažetak
Among animal diseases, colidiarrhea and colienterotoxemia caused by enterotoxigenic E. coli (ETEC) strains are the most prevalent porcine infections, commonly occurring in suckling and weaned pigs. The aim of this study was to test the immunogenicity and protective effectiveness of attenuated F18ac+ non-ETEC vaccine candidate strain against porcine colienterotoxemia by the quantitative phenotypic analysis of small intestinal leukocytes in weaned pigs. Additionally, we have evaluated adjuvanticity of levamisole as an immune response modifier. Following oral immunization with F18ac+ non-ETEC strain and challenge infection with F4ac+ ETEC strain, we have performed immunophenotipic localization and morphometric quantification of immune cell subsets in the small intestine of weaned pigs. The proportions of lymphoid CD3+, CD45RA+, CD45RC+, CD21+, IgA+ and myeloid SWC3+ cell subpopulations in the lamina propria and Peyer’ s patches of jejunum and ileum were evaluated by immunohistochemical avidin-biotin complex method and analyzed using software program Lucia G for digital image analysis. The numbers of these immune cells in the gut-associated lymphoid tissue were significantly increased in vaccinated/challenged pigs (p<0.05) compared to control nonvaccinated/challenge infected pigs. Numerically expressed data for jejunal/ileal cell subsets examined, demonstrated difference in capacity of protective immune responses between vaccinated and nonvaccinated, ETEC challenged pigs.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA