Evaluation of CD45+ cells kinetics in the blood of fattening chickens immunized with live or inactivated Newcastle disease vaccine

Popović, Maja; Balenović, Mirta; Ekert Kabalin, Anamaria; Savić, Vladimir; Vijtiuk, Nada; Ksenija, Vlahović; Valpotić, Ivica

Pregled bibliografske jedinice broj: 407174

Evaluation of CD45+ cells kinetics in the blood of fattening chickens immunized with live or inactivated Newcastle disease vaccine

Popović, Maja; Balenović, Mirta; Ekert Kabalin, Anamaria; Savić, Vladimir; Vijtiuk, Nada; Ksenija, Vlahović; Valpotić, Ivica

Evaluation of CD45+ cells kinetics in the blood of fattening chickens immunized with live or inactivated Newcastle disease vaccine // Veterinarski arhiv, 80 (2010), 1; 61-69 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 407174 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Evaluation of CD45+ cells kinetics in the blood of fattening chickens immunized with live or inactivated Newcastle disease vaccine

Autori
Popović, Maja ; Balenović, Mirta ; Ekert Kabalin, Anamaria ; Savić, Vladimir ; Vijtiuk, Nada ; Ksenija, Vlahović ; Valpotić, Ivica

Izvornik
Veterinarski arhiv (0372-5480) 80 (2010), 1; 61-69

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
cellular immunity ; flow cytometry ; fattening chickens ; Newcastle disease vaccine

Sažetak
Since insight into the effects of Newcastle disease vaccination on cellular immune responses has been insuffi cient to date, in this study the lymphocytes dynamics in the blood of fattening g chickens inoculated against Newcastle disease with lyophilised live vaccine of the La Sota virus strain (experimental group E1) and/or inactivated vaccine produced from the same strain (experimental group E2) were monitored. Blood was treated with labelled antibody for the CD45+ leukocyte marker and examined by fl ow cytometry. In both experimental groups a statistically signifi cant increase (P<0.01) of CD45+ cells was observed in relation to the control group of chickens, and the increase was more intensive and faster in the chickens vaccinated with lyophilised live vaccine than it was in chickens vaccinated with inactivated vaccine. The group treated with live vaccine had a signifi cantly increased number of CD45+ cells 6, 12, and 24 hr, and 7, 14, 21 and 28 days after inoculation. But on the 14th day after vaccination, a signifi cantly increased number of CD45+ cells (P<0.05) was found in the chickens inoculated with inactivated vaccine. It can be concluded that the lyophilised live vaccine was more effi cient and cellular immunity was achieved sooner than with the inactivated vaccine during the observed period of 28 days after inoculation.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA

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