Pregled bibliografske jedinice broj: 405135
THE ROLE OF GLUCAGON IN ACUTE ACETAMINOPHEN INDUCED HEPATOTOXICITY
THE ROLE OF GLUCAGON IN ACUTE ACETAMINOPHEN INDUCED HEPATOTOXICITY // 2008 Annual Meeting of the Croatian Immunological Society, Book of Abstracts / Vitale, Branko ; Rabatić, Sabina (ur.).
Zagreb: Hrvatsko imunološko društvo, 2008. str. 56-56 (poster, domaća recenzija, sažetak, znanstveni)
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Naslov
THE ROLE OF GLUCAGON IN ACUTE ACETAMINOPHEN INDUCED HEPATOTOXICITY
Autori
Kelava, Tomislav ; Ćavar, Ivan ; Pašalić, Marijan ; Čulo, Filip
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
2008 Annual Meeting of the Croatian Immunological Society, Book of Abstracts
/ Vitale, Branko ; Rabatić, Sabina - Zagreb : Hrvatsko imunološko društvo, 2008, 56-56
Skup
2008 Annual Meeting of the Croatian Immunological Society
Mjesto i datum
Šibenik, Hrvatska, 09.10.2008. - 12.10.2008
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
glucagon; NO; iNOS; acetaminophen; acute liver toxicity
Sažetak
The role of nitric oxide (NO) in hepatotoxicity induced by xenobiotics is still controversial. It is generally accepted that small, basal amount of NO, produced by constitutive NO synthase (cNOS) has a beneficial effect. However there are evidences that excessive production of NO from inducible NO synthase (iNOS) could be detrimental. Harbrecht et all. had shown that glucagon specifically inhibits iNOS expression and activity induced by LPS in rats. Therefore, we decided to investigate the effect of glucagon on acetaminophen (APAP) hepatotoxicity. In each experiment mice were divided into two groups, one group received glucagon (0, 5 mg/kg i.p.) 15-30 min before APAP and the other group received saline before APAP. To asses APAP (150 mg/kg i.p.) toxicity ALT levels in plasma were determined 6 and 24 hours following acetaminophen administration, or survival of mice were followed during 48 hours following APAP (300 mg/kg i.p.) administration. To asses iNOS activity plasma levels of nitrite/nitrate (the end products of NO metabolism) were determined 6 and 24 hours following APAP, using colorimetric assay based on the Griess reaction following the conversion of nitrate to nitrite using nitralyzer II kit. Mice in glucagon group had better survival and lower ALT levels at 6 and 24 hours after APAP, they also had lower plasma levels of nitrite/nitrate 6 hours after APAP, but there were no difference 24 hours after APAP. Currently we are determing iNOS expression in liver tissues.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
219-0000000-0328 - Uloga proupalnih citokina i prostaglandina u akutnom oštećenju jetre (Čulo, Filip, MZOS ) ( CroRIS)
Ustanove:
Medicinski fakultet, Zagreb