Naslov
Cytotoxicity of polyphenolic/flavonoid compounds in a leukemia cell culture

Autori
Josipović, Pavle ; Oršolić, Nada

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
50 godina molekularne biologije u Hrvatskoj / - , 2008

Skup
Znanstveni simpozij 50 godina molekularne biologije u Hrvatskoj

Mjesto i datum
Zagreb, Hrvatska, 20.11.2008. - 21.11.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
cytotoxicity; flavonoids; MOLT; JURKAT; HL-60; RAJI; U937cells

Sažetak
Flavonoid components of propolis are biologically active substances, with antioxidative, immunostimulative, immunomodulative and anti-inflamatory characteristics. The key intention of conducted studies was to prove their cytotoxic effect on different leukemia cell cultures. During research 5 different flavonoids have been used (quercetin, caffeic acid, chrysin, naringenin and naringin) and 5 types of leukemia cell cultures (MOLT, JURKAT, HL-60, RAJI and U937). Cells were cultured in RPMI-1640 medium, with the addition of 10% FCS, on temperature of 37º ; , in atmosphere with 5% of CO2. The above mentioned flavonoids were added in following concentrations (100, 50, 25, and/or 12.5 µ ; g mL-1). Result of flavonoids analysis showed different dose- and cell type -dependent cytotoxicity. Almost of flavonoids, quercetin induced strong cytotoxic effect in all tested cells. In addition caffeic acid and/or chrisyn also expressed high level of cytotoxicty. Treatment of U937 and HL-60 cell lines with low concentration of chrisyn and/or naringenin induced stimmulative effect on a cell proliferation ; the result suggested biphase effect of test component on monocyte cell lines. Cytotoxicity and growth stimulation mechanisms caused directly by flavonoids should be further investigated on molecular level.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Veterinarska medicina

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