Naslov
EFFECTOR CHANGES IN IMMUNE CELLS OF PEOPLE SITUATED NEAR GASOLINE INDUSTRY

Autori
Bulog, A, ; Mićović, V, ; Mrakovčić-Šutić, I,

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts / HID / Rabatić, Sabina et al. - Zagreb : Hrvatsko imunološko društvo, 2008

Skup
2008 ANNUAL MEETING OF THE CROATIAN IMMUNOLOGICAL SOCIETY

Mjesto i datum
Šibenik, Hrvatska, 09.10.2008. - 12.10.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
BTEX; environmental diseases; industrial area; innate immunity

Sažetak
Urban air pollutants, particularly benzene, toluene, etilbenzene, xilene (BTEX) and diesel exhaust particles contribute to the increasing incidence of many allergic and environmental diseases, as well as multiple chemical sensitivity or other related syndromes, what may result with an abnormal immune response based on environmental damage of lymphocyte subsets. The immune system has a capacity to differentiate innocuous and harmful foreign antigens, which are controlled by mechanisms of central and peripheral tolerance. The aim of this study was to examine alteration of cell mediate immunity and the role of cytolytic molecule perforin in different lymphocyte subpopulation in peripheral blood of inhabitants situated near gasoline industry compared with healthy donors. Venous blood samples were taken and phenotype of lymphocytes and perforin expression were analyzed using intracellular and surface immunofluoresecncy and flow cytometry (FACSCalibur). Statistical analysis was done in computer program Statistica 7.1 The results demonstrated that 17% of PBL in all examine group are perforin positive (total perforin is significantly lower in exposure than in control group). Percentages of double positive cells showed significant decrease in all examine lymphocyte subpopulations (CD3+ P+, CD4+P+, CD8+P+, CD16+P+ and CD56+P+ cells). Our data suggest that human perforin is differentially expressed in distinct sub-populations of peripheral blood lymphocytes of people exposure to gasoline and diesel, implicating the important role of perforin positive cells in innate immune response and cytotoxic activity of peripheral blood lymphocytes. Acknowledgement: This work was supported by grants from the Croatian Ministry of Science (No 0621341-0308 and 0620096-0094).

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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