Pregled bibliografske jedinice broj: 402181
POSTOPERATIVE PAIN MANAGEMENT TECHIQUES INFLUENCE ON THE EXPRESSION OF CYTOLITIC MOLECULE PERFORIN
POSTOPERATIVE PAIN MANAGEMENT TECHIQUES INFLUENCE ON THE EXPRESSION OF CYTOLITIC MOLECULE PERFORIN // Zbornik radova Prvog kongresa hrvatskih alergologa i kliničkih imunologa s međunarodnim sudjelovanjem / Asja Stipić-Marković (ur.).
Zagreb: Hrvatsko društvo za alergologiju i kliničku imunologiju ; Hrvatski liječnički zbor, 2009. (poster, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 402181 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
POSTOPERATIVE PAIN MANAGEMENT TECHIQUES INFLUENCE ON THE EXPRESSION OF CYTOLITIC MOLECULE PERFORIN
Autori
Golubović, Snježana ; Mrakovčić-Šutić, Ines ; Golubović, Vesna ; Sotošek Tokmadžić, Vlatka ; Petković, Marija
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Zbornik radova Prvog kongresa hrvatskih alergologa i kliničkih imunologa s međunarodnim sudjelovanjem
/ Asja Stipić-Marković - Zagreb : Hrvatsko društvo za alergologiju i kliničku imunologiju ; Hrvatski liječnički zbor, 2009
Skup
Prvi kongres hrvatskih alergologa i kliničkih imunologa s međunarodnim sudjelovanjem
Mjesto i datum
Zagreb, Hrvatska, 21.05.2009. - 23.05.2009
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
colorectal carcinoma; innate immunity; postoperative pain;
Sažetak
Background and aims: One of the common and very often, as well as the most difficult symptom in oncology patients is varying degrees of pain and this pain may cause the changes in immunological parameters of these patients. The pharmacological management of postoperative pain in patients with malignancies uses very different techniques whose possible cytotoxic features we still poorly understood. The present study compared two postoperative pain management techniques in patients undergoing colorectal carcinoma surgery: intravenous analgesia and patient-controlled epidural analgesia (PCEA), on expression of cytolitic molecule perforin in peripheral blood mononuclear cells of these patients. Methods: Venous blood samples were taken from colon carcinoma patients and also from healthy volunteers and peripheral blood leukocytes (PBL) were isolated. Phenotype of lymphocytes and perforin expression were analyzed using intracellular and surface immunofluoresecncy and flow cytometric analysis (FACSCalibur). Results: Our results demonstrated that 20-30% of PBL in all examine group are perforin positive (total perforin is significantly higher than in control group, but in patient underwent epidural analgesia these expression was higher than in intravenous experimental group). Conclusions: These preliminary data suggest that human perforin is differentially expressed in distinct sub-populations of peripheral blood lymphocytes of patients with colorectal carcinoma, implicating the important role of postoperative pain management techniques on expression of perforin positive cells and their role in naï ; ve immune response and cytotoxic activity of peripheral blood lymphocytes. Acknowledgment: This work was supported by grants from the Croatian Ministry of Science (projects: 0620096-0094 and 0620096-0092)
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti
POVEZANOST RADA
Projekti:
062-0620096-0092 - Epiduralna analgezija i stanična imunost u bolesnika s kolorektalnim karcinomom (Golubović, Vesna, MZOS ) ( CroRIS)
062-0620096-0094 - Regulacijske T i NKT stanice u kontroli tumorskog rasta, opeklina i autoimunosti (Mrakovčić-Šutić, Ines, MZOS ) ( CroRIS)
Ustanove:
Medicinski fakultet, Rijeka
Profili:
Marija Petković
(autor)
Ines Mrakovčić-Šutić
(autor)
Vesna Golubović
(autor)
Vlatka Sotošek
(autor)
Snježana Golubović
(autor)