Naslov
Haemolymph parameters and genotoxic response in freshwater crayfish Astacus leptodactylus after temperature stress

Autori
Lorenzon, Simonetta ; Malev, Olga ; Maguire, Ivana ; Šrut, Maja ; Štambuk, Anamaria ; Ferrero, Enrico A. ; Klobučar, Vinko Goran Igor

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
15th International Symposium on Pollutant Responses in Marine Organisms : Abstract Book / Stegeman, John J. ; Moore, Michael N. ; Garrigues, Philippe ; Minier, Christophe - Bordeaux, 2009, 207-207

Skup
International Symposium on Pollutant Responses in Marine Organisms (15 ; 2009)

Mjesto i datum
Bordeaux, Francuska, 17.05.2009. - 20.05.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
haemolymph; genotoxic; freshwater crayfish; Astacus leptodactylus; stress

Sažetak
In crustaceans changes in haemolymph parameters and genotoxic effects are likely indications of environmental disturbance. Their efficiency was already demonstrated in case of exposure to pollutants such as heavy metals and PCB. However, different environmental factors, such as temperature, should also be considered as potential inducers of stress and possible confounding factors in assessment of pollution effects. The aim of this work was to measure DNA damage in haemocytes of freshwater crayfish Astacus leptodactylus following temperature stress by means of the alkaline Comet assay and micronucleus test. Moreover, we have defined a set of suitable haemolymph parameters, and compared them with the genotoxic assays in terms of time-course response. Increased temperatures (25°C, 30°C), from the control temperature of 16± ; 1°C, induced already after 3 days a significant increase in DNA damage measured by the Comet assay. DNA damage returned to control levels within one week of recovery. Micronuclei induction showed a similar trend but did not reach the statistical significance. Concentration of glucose, lactic acid, total protein, pH and density of haemolymph in the groups of crayfish exposed to increased temperatures are significantly different from the control individuals since the first day of exposure. Our results proved the applicability of both sets of bioassays for the detection of stress in the freshwater crayfish A. leptodactylus caused by the increase in water temperature.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA