Naslov
T2 MAGNETIC RESONANCE SIGNAL OF THE CORPUS CALLOSUM REFLECTS GFAP POSITIVITY AND EXTRACELLULAR MATRIX CONTENT

Autori
Čuljat, Marko ; Jovanov-Milošević, Nataša ; Kostović, Ivica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract Book - NEUROGENOMICS AND NEUROIMAGING OF DEVELOPMENTAL DISORDERS / - , 2009

Skup
NEUROGENOMICS AND NEUROIMAGING OF DEVELOPMENTAL DISORDERS

Mjesto i datum
Dubrovnik, Hrvatska, 30.04.2009. - 05.05.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
corpus callosum; magnetic resonance; GFAP; extracellular matrix

Sažetak
Introduction: Many studies have correlated the magnetic resonance (MR) signal intensities with the histological data of the brain. These studies described periventricular migrating glial cells1 and cellular transitional fetal zones2. The internal structure of the corpus callosum (CC) is largely ignored in every-day clinical MR data analysis, describing only its size and general shape. Materials and methods: A formaldehyde-fixed neonatal brain was rinsed in phosphate buffered saline over night, and a T2 MR image was attained using the following parameters TR=8790, TE=92. The same brain was divided with a midsagittal cut, CC was isolated and embedded in paraffin. The paraffin slides were stained for GFAP immunocytochemistry3 (astrocyte markers) and PAS-Alcian histochemistry (extracellular staining). Results: Post mortem T2 weighted imaging of CC shows a laminar composition of CC. The ventral and dorsal surfaces have a decreased T2 signal, which form the border of CC as seen on MR imaging. The central part of the body and splenium have a characteristic railway-track appearance – two high signal intensity layers and sandwiched between them a low-signal intensity layer. In the genu the low T2 signal intensity is situated centrally. GFAP-positive network within CC is distributed in such a way as to mirror the low signal intensity distribution in the body and splenium, i.e. high positivity on dorsal and ventral surfaces and in the middle layer. Within the genu strong GFAP positivity is distributed in the central area. PAS-Alcian staining within the body and splenium is distributed in such a way as to oppose the distribution of GFAP positivity, i.e. dorsal and ventral surfaces are weakly stained as well as the central part, while the rest is stained slightly stronger. In the genu the staining is strong in the central area. Conclusion: The T2 signal intensity correlates well with the microstructure of CC, which sheds new light on the MR interpretation of CC.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA