Pregled bibliografske jedinice broj: 399388
Direct cytotoxicity of propolis and its polyphenolic compounds on primary culture of human papillary urothelial carcinoma cells, a possible use in therapy
Direct cytotoxicity of propolis and its polyphenolic compounds on primary culture of human papillary urothelial carcinoma cells, a possible use in therapy // Scientific evidence of the use of propolis in ehtnomedicine / Oršolić, Nada ; Bašić, Ivan (ur.).
Lahti: Transworld Research Network, 2008. str. 377-393
CROSBI ID: 399388 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Direct cytotoxicity of propolis and its polyphenolic compounds on primary culture of human papillary urothelial carcinoma cells, a possible use in therapy
Autori
Bašić, Ivan ; Oršolić, Nada ; Mihaljević, Željko ; Štajcar, Damir
Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, znanstveni
Knjiga
Scientific evidence of the use of propolis in ehtnomedicine
Urednik/ci
Oršolić, Nada ; Bašić, Ivan
Izdavač
Transworld Research Network
Grad
Lahti
Godina
2008
Raspon stranica
377-393
ISBN
978-81-7895-357-1
Ključne riječi
Propolis, Flavonoids, Human papillary urothelial carcinoma cells, Cytotoxicity, Primary culture
Sažetak
Chemoprevention of tumor with natural components, including propolis and propolis related polyphenolic/flavonoid compounds has recently drawn attention as a strong antitumor approach to be used. In case of recurrent non-muscle-involved urothelial carcinomas after transurethral resection intravesical instillation of chchemotherapy or BCG immunotherapy have mostly been employed. In the current study we investigated the direct cytotoxicity of propolis preparations and propolis polyphenolic/flavonoid compounds on papillary urothelial carcinoma cells in primary culture assay. A dose-depedent cytotoxicity was found when cells were exposed to either ethanolic (EEP) or water soluble preparation (WSDP) of propolis ; the polyphenolic/flavonoid compounds such as caffeic acid (CA), naringin (Nar), chrysin (Chrys), and quercetin (QU) exerted cytotoxicity to cancer cells in various degrees. The most active test components in this respect were EEP and QU respectively when the dose of 300 µ ; g/ml of culture was used regardless to cancer grade. At any time of expositure (24, 48, 72 hours) the efficacy of EEP and QU was statisticaly significant (p<0.05 ; p< 0.01). On the other hand, any of test components used exibited no cytotoxicity on normal epithelial bladder cells in primary culture. The cytotoxicity of test components was caracterized by morphological changes specific for apoptosis. Data suggest that propolis and propolis related polyphenolic/flavonoid compounds may be a promising treatment for papillary urothelial bladder cancer used as an adjuvant after transurethral resection.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti
POVEZANOST RADA
Projekti:
119-0000000-1255 - Kemoprevencija rasta tumora polifenolnim sastavnicama (Oršolić, Nada, MZOS ) ( CroRIS)
119-0532265-1254 - Imunoadjuvantno i zaštitno djelovanje propolisa u životinja (Benković, Vesna, MZOS ) ( CroRIS)
Ustanove:
Hrvatski veterinarski institut, Zagreb,
Prirodoslovno-matematički fakultet, Zagreb
