Pregled bibliografske jedinice broj: 39841
Fast Micromethod as a tool for DNA damage determination in different cell lines and tissues
Fast Micromethod as a tool for DNA damage determination in different cell lines and tissues // Silver Jubilee Meeting of the Croatian Biochemical Society / Flogel, Mirna (ur.).
Zagreb: Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 2000. (poster, nije recenziran, sažetak, znanstveni)
CROSBI ID: 39841 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Fast Micromethod as a tool for DNA damage determination in different cell lines and tissues
Autori
Jakšić, Željko ; Bihari, Nevenka ; Batel Renato
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Silver Jubilee Meeting of the Croatian Biochemical Society
/ Flogel, Mirna - Zagreb : Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 2000
Skup
Kongres hrvatskih biokemičara i molekularnih biologa uz međunarodno sudjelovanje HB 2000
Mjesto i datum
Zagreb, Hrvatska, 13.10.2000. - 15.10.2000
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
Fast Micromethod; DNA damage; cell lines; tissue
Sažetak
The induction of DNA damage was measured in native DNA isolated from cotton-spinner Holothuria tubulosa, marine sponge Suberites domuncula cells, mussel mytilus galloprovincialis solid tissue (gills) homogenate, Chinese Hamster Ovary cells (CHO), mouse muscle homogenate, human lymphocytes and human cell line (HeLa cells) by fast Micromethod. This method detects DNA damage (strand breaks, alkali.labile sites and incomplete excission repair) in cell suspension or tissue homogentaes in single microplates. Fast Micromethod requires very short time multiple analysis (less than 3 hours) and less than 10 ng DNA persingle well (about 3000 human, hamster or mouse cells, 12500 Suberites domuncula cells or about 25 ug of mussel gills homogenate). DNA damage could be detected after different doses of gama rays, generated by Cs137 (1800 Ci; 0-1500 rad) and UV-B light 254 nm (0-1000 J/m2) irradiation or chemical treatment by fifferent xenobiotics: NQO (4-nitroquinoline-N-oxide; 0-200 ng/ml), BaP (benzo(a)pyrene; 0-20 ug/g mussel) and Bleomycine (0-83 ug/ml). The method is sensitive to wide range of different doses and concentrations of DNA damaging agents. This makes it applicable for measurement of DNA integrity of small samoles for genotoxicty assessment as well as for analysisi related to human health and occupational exposure.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
