Pregled bibliografske jedinice broj: 397591
Determination of 8-hydroxy-2'deoxyguanosine in urine using HPLC with electrochemical detection
Determination of 8-hydroxy-2'deoxyguanosine in urine using HPLC with electrochemical detection // Arhiv za higijenu rada i toksikologiju, 59 (2008), 277-282 doi:10.2478/10004-1254-59-2008-1879 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 397591 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Determination of 8-hydroxy-2'deoxyguanosine in urine using HPLC with electrochemical detection
Autori
Domijan, Ana-Marija ; Peraica, Maja
Izvornik
Arhiv za higijenu rada i toksikologiju (0004-1254) 59
(2008);
277-282
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
biomarker; free radicals; oxidative DNA damage; 8-oxodG
Sažetak
Endogenous cell metabolism and different chemicals, drugs, ionising radiation, solar light, cigarette smoking, and air pollution can induce oxidative damage to DNA. Oxidative DNA damage is involved in the development of different diseases, aging, and cancer. Urine 8-hydroxy-2'deoxyguanosine (8-oxodG) is widely accepted as a biomarker of the "whole body" oxidative DNA damage. The aim of this study was to introduce a method for determination 8-oxodG in spot urine samples using high-performance liquid chromatography with electrochemical detection. In the tested range of 50-400 nM, the calibration curve was linear (r2= 0.9995) and the limit of detection was 3 nM. Concentrations of 8-oxodG ranged between 3.0 nM and 48.0 nM, which is in accordance with values reported for healthy individuals in other studies.
Izvorni jezik
Engleski
Znanstvena područja
Matematika, Kemija, Arhitektura i urbanizam
POVEZANOST RADA
Projekti:
022-0222148-2142 - Toksični učinci mikotoksina na ljude i životinje (Peraica, Maja, MZOS ) ( CroRIS)
Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
Uključenost u ostale bibliografske baze podataka::
- MEDLINE
