Naslov
H-ras gene polymorphisms and sporadic colon cancer in Croatian population

Autori
Catela Ivković, Tina ; Kapitanović, Sanja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
European Journal of Human Genetics, European Human Genetics Conference 2009 / ESHG - Beč : Nature publishing group, 2009, 174-174

Skup
European Human Genetics Conference 2009

Mjesto i datum
Beč, Austrija, 23.05.2009. - 26.05.2009

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
H-ras; polymorphisms; sporadic colon cancer

Sažetak
High incidence of colon cancer worldwide indicates the importance of exploring genetic alterations that lead to its carcinogenesis. Two polymorphisms in H-ras gene, hexanucleotide tandem repeats in the first intron and SNP T81C in the first exon, that might be connected with susceptibility to cancer have been described. The aim of our study was to investigate these loci in Croatian population and to determine if any of them is connected with susceptibility to colon cancer. Two hundred healthy volunteers and 200 colon cancer patients were genotyped using PCR and RFLP methods. Frequencies at hexanucleotide locus were 44, 5%, 22, 5%, 5, 5%, 7%, 1% and 19, 5% in healthy population, and 49%, 26%, 3, 5%, 2, 5%, 5, 5% and 13, 5% in colon cancer for P1/P1, P1/P2, P2/P2, P2/P3, P3/P3 and P1/P3 genotype respectively. Allele frequencies were 65, 5%, 20, 3% and 14, 2% in healthy population and 68, 8%, 17, 8% and 13, 5% in colon cancer for P1, P2 and P3 respectively. For SNP T81C locus frequencies were 42, 5%, 42, 5% and 15% in healthy population and 62, 5%, 35% and 2, 5% in colon cancer for TT, TC and CC respectively. Allele frequencies were 63, 8 % and 36, 2% in healthy population and 80% and 20% in colon cancer for T and C alleles respectively. Genotype P3/P3 was more common in colon cancer patients and P2/P2 and P2/P3 genotypes in healthy population. Allele -81C was more common in healthy volunteers in comparison to colon cancer patients.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA