Naslov
Induction of micronuclei in human lymphocytes after occupational exposure to ultrasound

Autori
Garaj-Vrhovac, Vera ; Kopjar, Nevenka ; Besendorfer, Višnja ; Papeš, Dražena

Izvornik
Chemosphere (0045-6535) 38 (1999), 15; 3541-3553

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
ultrasound; occupational exposure; human lymphocytes; micronuclei

Sažetak
Micronucleus assay combined with Giemsa and DAPI staining was performed on blood samples of subjects occupationally exposed ultrasound. Lymphocytes were cultivated in vitro for 72 h. At 44 h cytichalasin-B was added in cultures. Frequencies of micronuclei in exposed subjects statistically significant increased compared to control. The frequency of micronucleated cells and micronuclei in exposed subjects shows interindividual variability. Using DAPI staining we observed signal-positive and signal-negative micronuclei. Percentage of signal-positive micronuclei varies between 0 and 66.7% and signal-negative micronuclei between 33.3% and 100%. This study indicate harmful effects of ultrasound on human genome, but further investigations are necessary.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA