Naslov
Perforin expression changes in peripheral blood lymphocytes of patients with burn injuries

Autori
Pirjavec-Mahić, Aleksandra ; Mrakovčić-Šutić, Ines

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
14th Congress of the International Society for Burn Injuries : Book of abstracts / - , 2008

Skup
Congress of the International Society for Burn Injuries /14 ; 2008)

Mjesto i datum
Montréal, Kanada, 07.09.2008. - 11.09.2008

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
burns; cytotoxicity; immune response; perforin;

Sažetak
Burn injuries can induce accelerated lymphocyte apoptosis in reconvalescent period. This accelerated lymphocyte depletion can be associated with high risk of complications, especially infections. Cytotoxic T cells (CTL) and natural killer (NK) cells induce cell death of their target cells by the release of perforin and granzymes or by the surface interaction between Fas ligand and Fas. The mean idea of this study was to examine alteration of cell mediate immunity and cytolytic molecule perforin in different lymphocyte subpopulation in peripheral blood of burned patients. Peripheral blood leukocytes were isolated on density gradient from burned patients and from healthy volunteers. Phenotype of lymphocytes and perforin expression were analyzed using intracellular and surface immunofluoresecncy and flow cytometry (FACSCalibur). Statistical analysis was done in computer program Statistica 7.1 (StatSoft, Inc., Tulsa, OK, USA). Graphs and tables were done in computer program Microsoft Excel. Our data demonstrated that only 10-15% of PBL in patients are perforin positive (total perforin of burned patients are 40% lower than in control group). Percentage of all double positive cells showed significant decrease (CD3+ P+ , CD4+P+, CD8+P+, CD16+P+, CD56+P+) in patients with burns injuries in comparation with healthy volunteers, showing the possible prognostic recovery value of these findings. Our results suggest that human perforin is differentially expressed in distinct sub-populations of peripheral blood lymphocytes of our patients, indicating the important role of perforin positive cells in naï ; ve immune response and cytotoxic activity of peripheral blood cells of patients with burn injuries. This work was supported by grant from the Croatian Ministry of Science (project 0620096-0094).

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

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