Pregled bibliografske jedinice broj: 395320
Comparison of postoperative pain management techniques on expression of cytolitic molecule perforin
Comparison of postoperative pain management techniques on expression of cytolitic molecule perforin // Pain Practice, vol 9, Supp. 1, 2009 / Craig T. Hartrick (ur.).
Texas City (TX): Texas Pain Society, 2009. str. 98-99 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 395320 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Comparison of postoperative pain management techniques on expression of cytolitic molecule perforin
Autori
Golubović, Vesna ; Mrakovčić-Šutić, Ines ; Golubović, Snježana ; Sotošek Tokmadžić, Vlatka ; Petković, Marija
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Pain Practice, vol 9, Supp. 1, 2009
/ Craig T. Hartrick - Texas City (TX) : Texas Pain Society, 2009, 98-99
Skup
5th World Congress-World Institute of Pain
Mjesto i datum
Sjedinjene Američke Države, 13.03.2009. - 14.03.2009
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
intravenous analgesia; pain; perforin; postoperative epidural analgesia
Sažetak
Background and aims: The pharmacological management of postoperative pain in patients with malignancies uses very different techniques whose possible cytotoxic functions we still known very poor. The present study compared two postoperative pain management techniques in patients undergoing intravenous analgesia and patient-controlled epidural analgesia (PCEA), on expression of cytolitic molecule perforin in peripheral blood mononuclear cells of patients after colorectal carcinoma operation. Methods: Venous blood samples were taken from colon carcinoma patients and healthy volunteers and peripheral blood leukocytes (PBL) were isolated. Phenotype of lymphocytes and perforin expression were analyzed using intracellular and surface immunofluoresecncy and flow cytometric analysis (FACSCalibur). Results:Our results demonstrated that 20-30% of PBL in all examine group are perforin positive (total perforin is significantly higher than in control group, but in patient underwent epidural analgesia these expression was higher than in intravenous experimental group). Conclusions: These preliminary data suggest that human perforin is differentially expressed in distinct sub-populations of peripheral blood lymphocytes of patients with colorectal carcinoma, implicating the important role of postoperative pain management techniques on expression of perforin positive cells and their role in naï ; ve immune response and cytotoxic activity of peripheral blood lymphocytes. Acknowledgment: This work was supported by grants from the Croatian Ministry of Science (projects: 0620096-0094 and 0620096-0092)
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti
POVEZANOST RADA
Projekti:
062-0620096-0092 - Epiduralna analgezija i stanična imunost u bolesnika s kolorektalnim karcinomom (Golubović, Vesna, MZOS ) ( CroRIS)
062-0620096-0094 - Regulacijske T i NKT stanice u kontroli tumorskog rasta, opeklina i autoimunosti (Mrakovčić-Šutić, Ines, MZOS ) ( CroRIS)
Ustanove:
Medicinski fakultet, Rijeka
Profili:
Marija Petković
(autor)
Vesna Golubović
(autor)
Ines Mrakovčić-Šutić
(autor)
Vlatka Sotošek
(autor)
Snježana Golubović
(autor)