Naslov
Wistar-Zagreb 5HT rats: a rodent model with constitutional upregulation/downregulation of serotonin transporter

Autori
Jernej, Branimir ; Čičin-Šain, Lipa

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings of the 12-th Multidisciplinary International Neuroscience and Biological Psychiatry Conference "Stress and Behavior" / Kalueff, Allan V ; Egan, Rupert J - Sankt Peterburg : Centre for Physiology and Biochemical Research, 2009, 7-7

Skup
12-th Multidisciplinary International Neuroscience and Biological Psychiatry Conference "Stress and Behavior"

Mjesto i datum
Sankt Peterburg, Ruska Federacija, 16.05.2009. - 20.05.2009

Vrsta sudjelovanja
Plenarno

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Wistar-Zagreb 5HT rat; serotonin; platelet; brain; selection

Sažetak
Introduction and Methods: By selective breeding for the extreme values of platelet serotonin level (PSL), two sublines of rats with constitutional hyperserotonemia/hyposerotonemia were developed. The velocity of platelet serotonin uptake (PSU), the main determinant of PSL, was used as a further, more specific, selection criterion and directed breeding for its extremes resulted in two sublines of rats with constitutional up-regulation/down-regulation of platelet 5HT transporter activity, and consequent alterations of entire 5HT homeostasis. These sublines, termed Wistar-Zagreb 5HT rats (WZ-5HT rats, constitute a genetic rodent model described in this chapter. Results and Discussion: Besides changes in peripheral 5HT homeostasis, high-5HT and low-5HT sublines of WZ-5HT rats also demonstrate changes in central serotonergic mechanisms. Under physiological conditions, neurochemical differences in the 5HT system between sublines were almost undetectable, but they become evident upon specific pharmacologic challenge, as shown by brain microdialysis study. Differential behavioral phenotypes of 5HT sublines in response to various environmental challenges provide further evidence for differences in their brain functioning. Thus, rats with high-5HT levels exhibit enhanced anxiety-like behaviors while depressive-like behavior and higher alcohol intake co-occur in low 5HT animals. Observed functional and behavioral differences between sublines of WZ-5HT rats strongly indicate that the brain serotonergic activity is increased in animals from the high-5HT subline as compared to low-5HT rats. Conclusion: The WZ-5HT rat model may represent an integrative model for serotonin transporter (and serotonin in general) research, incorporating changes at genomic/genetic and phenotypis (neurodevelopmental, structural, biochemical, behavioral, etc.) levels and encompassing both central and peripheral 5HT functioning.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA