Naslov
FT-IR spectroscopy of lipoproteins — A comparative study

Autori
Krilov, Dubravka ; Balarin, Maja ; Kosović, Marin ; Gamulin, Ozren ; Brnjas-Kraljević, Jasminka

Izvornik
Spectrochimica Acta Part A - Molecular and Biomolecular Spectroscopy (1386-1425) 73 (2009), 4; 701-706

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Lipoprotein; Apolipoprotein; Lipid; FT-IR spectroscopy

Sažetak
FT-IR spectra, in the frequency region 4000– 600 cm− 1, of four major lipoprotein classes: very low density lipoprotein (VLDL), low density lipoprotein (LDL) and two subclasses of high density lipoproteins (HDL2 and HDL3)were analyzed to obtain their detailed spectral characterization. Information about the protein domain of particle was obtained from the analysis of amide I band. The procedure of decomposition and curve fitting of this band confirms the data already known about the secondary structure of two different apolipoproteins: apo A-I in HDL2 and HDL3 and apo B-100 in LDL and VLDL. For information about the lipid composition and packing of the particular lipoprotein the well expressed lipid bands in the spectra were analyzed. Characterization of spectral details in the FT-IR spectrum of natural lipoprotein is necessary to study the influence of external compounds on its structure.

Izvorni jezik
Engleski

Znanstvena područja
Fizika

POVEZANOST RADA