Naslov
Decreased Toll-like receptor 8 expression and lower TNF-alpha synthesis in infants with acute RSV infection

Autori
Bendelja, Krešo ; Vojvoda, Valerija ; Aberle, Neda ; Čepin-Bogović, Jasna ; Gagro, Alenka ; Mlinarić- Galinović, Gordana ; Rabatić, Sabina

Izvornik
Respiratory research (1465-9921) 11 (2010), 10; 143-154

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
toll-like receptors ; TLR8 ; respiratory syncytial virus ; RSV ; infants

Sažetak
Toll-like receptors (TLRs) are part of the innate immune system, able to recognize pathogen- associated molecular patterns and activate immune system upon pathogen challenge. Respiratory syncytial virus (RSV) is a RNA virus particularly detrimental in infancy. It could cause severe lower respiratory tract disease and recurrent infections related to inadequate development of anti-viral immunity. The reason could be inadequate multiple TLRs engagement, including TLR8 in recognition of single-stranded viral RNA and diminished synthesis of inflammatory mediators due to a lower expression. Intracellular TLR8 expression in peripheral blood monocytes from RSV- infected infants was profiled and compared to healthy adults and age matched controls. Whether the observed difference in TLR8 expression is a transitory effect, infants in convalescent phase (4-6 weeks later) were retested. Specific TLR8- mediated TNF-alpha production in monocytes during an acute and convalescent phase was analyzed. RSV- infected and healthy infants had lower percentage of TLR8-expressing monocytes than healthy adults whereas decreased of TLR8 protein levels were detected only for RSV-infected infant group. Lower protein levels of TLR8 in monocytes from RSV- infected infants, compared to healthy infants, negatively correlated with respiratory frequency and resulted in lower TNF-alpha synthesis upon a specific TLR8 stimulation. In the convalescent phase, levels of TLR8 increased, accompanied by increased TNF-alpha synthesis compared to acute infection.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA