Naslov
Comparison of protocols for measuring activities of human blood cholinesterases by the Ellman method

Autori
Reiner, Elsa ; Šinko, Goran ; Škrinjarić-Špoljar, Mira ; Simeon-Rudolf, Vera

Izvornik
Arhiv za higijenu rada i toksikologiju (0004-1254) 51 (2000), 1; 13-18

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
acetylcholinesterase; butyrylcholinesterase; human erythrocytes; human plasma

Sažetak
This paper presents a protocol for routine assays of human blood cholinesterase activities which separates erythrocytes from plasma by centrifugation and measures acetylcholinesterase activity in unwashed erythrocytes and butyrylcholinesterase activity in the plasma. The recommended substrate for both enzymes is 1.0 mM acetylthiocholine. The protocol is compared with other two recommended protocols for the activity measurements of the two enzymes using the Ellman method. The paper discusses the adventages and disadventages of each and concludes with a proposal for an international agreement between laboratories for the evaluation of a standardized protocol.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA