Naslov
Acetylcholinesterase and butyrylcholinesterase activity measurements in human blood by the Ellman method: II. Procedure protocol and comparison with procedures suggested by other authors

Autori
Reiner, Elsa ; Škrinjarić-Špoljar, Mira ; Šinko, Goran ; Simeon-Rudolf, Vera

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Technical Program, CB Medical Treatment Symposium: An Exploration of Present Capabilities and Future Requirements for Chemical and Biological Medical Treatment, Spiez, Abstracts of Platform and Poster Presenations / Price, Barbara - Spiez : AC-Laboratorium Spiez, Švicarska, 2000

Skup
The Third Chemical and Biological Medical Treatment Symposium, Spiez, Švicarska

Mjesto i datum
Spiez, Švicarska, 07.05.2000. - 15.05.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
acetylcholinesterase; butyrycholinesterase; activity measurement; human blood

Sažetak
The procedure for plasma butyrylcholinesterase (BChE ; EC 3.1.1.8) activity measurement is well established, while this is not the case for the erythrocyte acetylcholinesterase (AChE ; EC 3.1.1.7). We suggest the separation of plasma from erythrocytes by centrifugation, followed by separate measurement (with 1.0 mM acetylthiocholine as substrate) of BChE in plasma and AChE in unwashed erythrocytes. The obtained results showed that the cholinesterase activity measured in whole blood equalled the summe of the AChE activity in suspended erythrocytes plus the BChE activity in plasma, calculated upon the measured haematocrit. In blood samples from 13 individuals, the summe of activities was 97 ą 7 % of the activity measured in whole blood. The above procedure was compared with the method of Portmann (CBMTS, Dubrovnik 1998) based on activity measurements with acetylthiocholine and butyrylthiocholine which distinguish AChE from BChE, and with the method of Szinicz et al. (CBMTS, Dubrovnik 1998) where AChE activities are measured with acetylthiocholine in presence of ethopropazine, a BChE inhibitor. The advantage of our procedure is that both enzymes are measured with the same substrate and no inhibitor is required for the AChE and BChE assays. Further, the procedure can be performed with commercial kits for plasma activity measurements.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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