Pregled bibliografske jedinice broj: 389073
Perforin expression in skin lesions of psoriatic patients
Perforin expression in skin lesions of psoriatic patients // 10th Congress of European Academy of Dermatology and Venereology
Prag, Češka Republika, 2002. (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 389073 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Perforin expression in skin lesions of psoriatic patients
Autori
Prpić Massari, Larisa ; Kaštelan, Marija ; Gruber, Franjo ; Zamolo, Gordana ; Jonjić, Nives ; Rukavina, Daniel
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Skup
10th Congress of European Academy of Dermatology and Venereology
Mjesto i datum
Prag, Češka Republika, 02.10.2002. - 06.10.2002
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
Psoriasis; Cell mediated cytotoxicity; NK mediated cytotoxicity; Perforin; Skin lesions
(psoriasis; cell mediated cytotoxicity; NK mediated cytotoxicity; perforin; skin lesions)
Sažetak
Psoriasis is T cell mediated autoimmune skin disease characterized by infiltration of inflammatory cells, especially T lymphocytes and production of inflammatory cytokines, mostly of Th1 type. Oscillations in phenotype of T lymphocytes are observed in peripheral blood (PB) and skin lesions, depending on stage of disease. CD4+ T cell predominance in exacerbated psoriatic plaques and CD8+ T cells in resolutive lesions are demonstrated. Cell mediated cytotoxicity reactions (CMC) are reactions mediated by secretion of cytolytic molecule perforin and granzyme or binding of FasL (CD95L) with Fas receptor (CD95) on target cell. CD8+ T lymphocytes and to a certain degree CD4+ T lymphocytes as well as NK cells mediate in CMC. Perforin (P) is cytolytic molecule expressed in the granules of cytolytic cells both CTL and NK cells, released in the contact with the target cells subsequently exerting either necrotic or apoptotic (in collaborations with serine esterases) cell death. Our former results point on participation of cytolytic mechanisms in immunopathogenesis of psoriasis. Perforin expression is upregulated in peripheral blood T lymphocytes (especially CD8 phenotype) and CD56+ NK cells in exacerbation phase of psoriasis vulgaris compared to remission. Thus, role of cytolytic pathway mediated by perforin is recognized in psoriasis in PBL and in lesions but still insufficiently defined. The aim of our new investigation was to analyze expression of perforin in the psoriatic skin lesions and highlight a role of perforin mediated cytotoxicity in pathophysiology of disease. Skin biopsies of eight psoriatic patients, four in exacerbation and four in remission phase of psoriasis vulgaris were investigated. They were obtained from inside border of stable plaque (in a case of remission) and newly exacerbating plaque (exacerbating phase) and from uninvolved skin of the same patients biopsied at least 3 cm away of affected skin. Single immunoenzyme staining was performed using biotin-streptavidin-peroxidase method. Our preliminary results showed stronger perforin expression in involved psoriatic skin in exacerbating phase compared to remission. The same tendency of increasing number of cells in exacerbation phase was noticed among CD56+ NK population. These results point on the potential role of cytolytic mechanisms mediated by perforin compared to remission of disease.
Izvorni jezik
Engleski
Znanstvena područja
Kliničke medicinske znanosti
POVEZANOST RADA
Projekti:
0062040
Ustanove:
Medicinski fakultet, Rijeka,
Klinički bolnički centar Rijeka
Profili:
Larisa Prpić Massari
(autor)
Nives Jonjić
(autor)
Daniel Rukavina
(autor)
Franjo Gruber
(autor)
Marija Kaštelan
(autor)
Gordana Zamolo
(autor)