In vitro cultivation of porcine limbal stem cells

Popović, Maja; Tominac, Mirna; Vlahović, Ksenija; Kezić, Dubravko; Šperanda, Marcela; Grabarević, Željko; Popović, Iva; Pirkić, Boris; Šeol, Branka; Valpotić, Ivica

doi:10.4081/ijas.2009.s3.125

Pregled bibliografske jedinice broj: 389013

In vitro cultivation of porcine limbal stem cells

Popović, Maja; Tominac, Mirna; Vlahović, Ksenija; Kezić, Dubravko; Šperanda, Marcela; Grabarević, Željko; Popović, Iva; Pirkić, Boris; Šeol, Branka; Valpotić, Ivica

In vitro cultivation of porcine limbal stem cells // Italian journal of animal science, 8 (2009), S3; 125-127 doi:10.4081/ijas.2009.s3.125 (međunarodna recenzija, kratko priopcenje, znanstveni)

CROSBI ID: 389013 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
In vitro cultivation of porcine limbal stem cells

Autori
Popović, Maja ; Tominac, Mirna ; Vlahović, Ksenija ; Kezić, Dubravko ; Šperanda, Marcela ; Grabarević, Željko ; Popović, Iva ; Pirkić, Boris ; Šeol, Branka ; Valpotić, Ivica

Izvornik
Italian journal of animal science (1594-4077) 8 (2009), S3; 125-127

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kratko priopcenje, znanstveni

Ključne riječi
Stem cells ; porcine limbus ; cultivation

Sažetak
Similarly as in other organ structures stem cells are present in cornea residing basal epithelial layer termed palisade of Voight. A growing interest in allografts and xenografts implies a thorough study of regenerative potentials of these cells as well as a clear description of their patterns in in vitro tissue cultures to be grafted. Recently we have developed a simple method for cultivation of porcine corneal epithelial stem cells obtained by biopsy from the limbal region. Eight enucleated eyes were obtained from four slaughtered pigs. A 5mm2 samples of limbal epithelium were taken by keratotomy method. The primary cultures of these cells showed phenotypic and morphometric characteristics of porcine corneal epithelial cells following May- Grunwald-Giemsa staining. After 5 days of sowing they reached 80% of confluence. With the « Night & Day» lenses a total confluence was achieved 5 days earlier in comparison to the cells that were grown in the secondary cultures. Accordingly, the use of porcine limbal stem cells has potentials in veterinary medicine (as novel approach in reparative/regenerative medicine of pets, horses and selected breeds of food animals), but also are in accordance with the extensive studies on the potential use of xenografts, mainly swine tissues/organs, in humans.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

Napomena
Proceedings of the 17th International Symposium
Animal Science Day "Priorities for the European
animal production in a global market", Session
1 : Animal Breeding, Conservation and
Biotechnology ; Abano Terme, Padova, Italija,
15.-18.09.2009.

POVEZANOST RADA

Projekti:
MZOS-053-0481153-1129 - Mikoplazmoze i neke uvjetovane infekcijske bolesti životinja (Šeol, Branka, MZOS ) ( CroRIS)
MZOS-053-0531863-1861 - Klamidioza ptica i sisavaca (Vlahović, Ksenija, MZOS ) ( CroRIS)
MZOS-053-0532264-2260 - Komparativna dijagnostika, morfometrija i analiza tumora ljudi i životinja (Grabarević, Željko, MZOS ) ( CroRIS)
MZOS-053-0532265-2242 - Utjecaj biotehnoloških postupaka na zdravlje, reprodukciju i dobrobit svinja (Pavičić, Željko, MZOS ) ( CroRIS)
MZOS-053-0532265-2255 - Kolidiareja i kolienterotoksemija prasadi : mukozna imunost i imunomodulacija (Popović, Maja, MZOS ) ( CroRIS)
MZOS-079-0793448-3438 - Prirodni imunomodulatori i antioksidansi u optimalizaciji proizvodnje svinja (Šperanda, Marcela, MZOS ) ( CroRIS)