Naslov
Temporal association between lens protein glycation and cataract development in diabetic rats

Autori
Turk, Zdenka ; Mišur, Irena ; Turk, Nikša

Izvornik
Acta diabetologica (0940-5429) 34 (1997), 1; 49-54

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
diabetes; diabetic complications; glycation process; cataracogenesis

Sažetak
In an attempt to shed more light on the relation between the glycation process and structural protein alterations, we followed the formation of glycated products in the lenses of hyperglycaemic Wistar rats during a period of 5 months following alloxan diabetes inducement. The study groups included non-diabetic (control), untreated diabetic rats (D), and diabetic rats receiving insulin alone or in combination with phlorizin, an inhibitor of renal tubular glucose transport. Lenses were removed at 4 and 20 weeks, and advanced glycation products in alkali-soluble lens proteins were determined by their characteristic spectrofluorescence (emission at 385 nm with excitation of 335 nm). In 20-week untreated diabetic rats as compared to control rats, a significant increase was observed in the fluorescence level (3.25+/-1.02 vs 1.61+/-0.17 FU/mg, P

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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