Naslov
Seminal plasma and sperm- bound lactate dehydrogenase LDH-C4 activity in infertile men
(Seminal plasma andsperm-bound lactate dehydrogenase LDH-C4 in infertile men)

Autori
Gavella, Mirjana ; Lipovac, Vaskresenija ; Cvitković, Petar ; Čolak, Božo

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
International Journal of Andrology, Supplement 1 / Frick, J. - : Wiley-Blackwell, 1997

Skup
VIInternational Congress of Andrology

Mjesto i datum
Salzburg, Austrija, 25.05.1997. - 29.05.1997

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
lactate dehydrogenase LDH-C4; spermatozoa; seminal plasma; infertile men

Sažetak
Monitoring of biochemical markers in infertile men might allow identification of some testicular function disturbances. Appearance of sperm-specific isoenzyme LDH-C4 in seminal plasma has received attention since seminal plasma LDH-C4 activi8ty expressed per 10 6 spermatozoa probably relates to the degree of degeneration of earlier germ-cells types within the testis. On the other hand, it has been found that the increase in LDH-C4 activity in spermatozoa is probably due to impaired cytoplasmic elimination and/or the presence of immature spermatozoa with unextruded cytoplasm. The aim of this study was to establish whether concurrent measurement of LDH-C4 activity in seminal plasma and in spermatozoa of infertile men has any practical application in the examination of male infertility. With our own modification of automated assay , we simultaneously measured seminal plasma and sperm bound LDH-C4 activities in 56 semen samples on an Abbott ABA-100 bichromatic spectrophotometer, using 2-oxohexanoate as a substrate, with a precision CV=5.2%. Both enzymatic activities were found to be significant higher in oligozoospermic samples (below 20 x 10 6 spermatozoa/ml). Median for sperm-bound LDH-C4 in 24 oloigozoospermic samples amounted to 52.5 mIU/million spermatozoa as compared to 26.6 mIU/million spermatozoa in 32 normozoospermic men with above 20 mill. spermatozoa/ml (p 0.05). LDH C4 activity/sperm ratio in seminal plasma was 17.4 in oligozoospermia vs 8.1 in normozoospermia (p 0.0001). Correlation analysis showed the seminal plasma LDH-C4 activity to be significantly related to the sperm-bound LDH_C4 activity (p 0.001) in normozoospermic men, whereas this relationship was less evident in oligozoospermic men (p 0.05). This suggests the necessity of simultaneous determination of both sperm and seminal plasma LDH-C4 activities in oligozoospermic men to evaluatenone of the possible causes of their infertility.

Izvorni jezik
Engleski

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