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Pregled bibliografske jedinice broj: 386744

Isolation of cell-free DNA from plasma by chromatography on short monolithic columns and quantification of non-apoptotic fragments by real-time polymerase chain reaction

Ivančić-Jelečki, Jelena; Brgles, Marija; Šantak, Maja; Forčić, Dubravko
Isolation of cell-free DNA from plasma by chromatography on short monolithic columns and quantification of non-apoptotic fragments by real-time polymerase chain reaction // Journal of Chromatography A, 1216 (2009), 13; 2717-2724 doi:10.1016/j.chroma.2008.10.087 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 386744 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Isolation of cell-free DNA from plasma by chromatography on short monolithic columns and quantification of non-apoptotic fragments by real-time polymerase chain reaction

Autori
Ivančić-Jelečki, Jelena ; Brgles, Marija ; Šantak, Maja ; Forčić, Dubravko

Izvornik
Journal of Chromatography A (0021-9673) 1216 (2009), 13; 2717-2724

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cell-free DNA; DNA isolation; Chromatography; Plasma; Convective Interaction Media (CIM); Quantitative PCR

Sažetak
Human plasma is an important medical substance and a raw material for production of various therapeutics. During blood sampling, storage and processing, genomic DNA is released into plasma from nucleated blood cells that are damaged in the course of the procedure. In order to determine the concentration of contaminating DNA in plasma, we developed a method for DNA isolation by using anion-exachange chromatography on a BIA Separations CIM (convective interaction media) diethylaminoethyl column. DNA was quantified by SYBR Green based real-time polymerase chain reaction. The concentration of cell-free, non-apoptotic DNA in plasma ranged between 0.06 and 22.5 ng/ml. As substantial volumes of plasma or whole blood are administered directly into the vascular system, a recipient is exposed to high amounts of cell-free DNA, several orders of magnitude higher than the amount found in other biologicals.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti



POVEZANOST RADA

Projekti:
021-0212432-3123 - Molekularna patogeneza virusa mumpsa (Šantak, Maja, MZOS ) ( CroRIS)

Ustanove:
Imunološki zavod d.d.

Profili:

Avatar Url Maja Šantak (autor)

Avatar Url Jelena Ivančić-Jelečki (autor)

Avatar Url Marija Brgles (autor)

Avatar Url Dubravko Forčić (autor)

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com

Citiraj ovu publikaciju:

Ivančić-Jelečki, Jelena; Brgles, Marija; Šantak, Maja; Forčić, Dubravko
Isolation of cell-free DNA from plasma by chromatography on short monolithic columns and quantification of non-apoptotic fragments by real-time polymerase chain reaction // Journal of Chromatography A, 1216 (2009), 13; 2717-2724 doi:10.1016/j.chroma.2008.10.087 (međunarodna recenzija, članak, znanstveni)
Ivančić-Jelečki, J., Brgles, M., Šantak, M. & Forčić, D. (2009) Isolation of cell-free DNA from plasma by chromatography on short monolithic columns and quantification of non-apoptotic fragments by real-time polymerase chain reaction. Journal of Chromatography A, 1216 (13), 2717-2724 doi:10.1016/j.chroma.2008.10.087.
@article{article, author = {Ivan\v{c}i\'{c}-Jele\v{c}ki, Jelena and Brgles, Marija and \v{S}antak, Maja and For\v{c}i\'{c}, Dubravko}, year = {2009}, pages = {2717-2724}, DOI = {10.1016/j.chroma.2008.10.087}, keywords = {Cell-free DNA, DNA isolation, Chromatography, Plasma, Convective Interaction Media (CIM), Quantitative PCR}, journal = {Journal of Chromatography A}, doi = {10.1016/j.chroma.2008.10.087}, volume = {1216}, number = {13}, issn = {0021-9673}, title = {Isolation of cell-free DNA from plasma by chromatography on short monolithic columns and quantification of non-apoptotic fragments by real-time polymerase chain reaction}, keyword = {Cell-free DNA, DNA isolation, Chromatography, Plasma, Convective Interaction Media (CIM), Quantitative PCR} }
@article{article, author = {Ivan\v{c}i\'{c}-Jele\v{c}ki, Jelena and Brgles, Marija and \v{S}antak, Maja and For\v{c}i\'{c}, Dubravko}, year = {2009}, pages = {2717-2724}, DOI = {10.1016/j.chroma.2008.10.087}, keywords = {Cell-free DNA, DNA isolation, Chromatography, Plasma, Convective Interaction Media (CIM), Quantitative PCR}, journal = {Journal of Chromatography A}, doi = {10.1016/j.chroma.2008.10.087}, volume = {1216}, number = {13}, issn = {0021-9673}, title = {Isolation of cell-free DNA from plasma by chromatography on short monolithic columns and quantification of non-apoptotic fragments by real-time polymerase chain reaction}, keyword = {Cell-free DNA, DNA isolation, Chromatography, Plasma, Convective Interaction Media (CIM), Quantitative PCR} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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