Naslov
Novel morphological and genetic markers for the discrimination of three European Pityokteines (Coleoptera: Curculionidae: Scolytinae) species

Autori
Pernek, Milan ; Avtzis, Dimitrios N. ; Hrašovec, Boris ; Diminić, Danko ; Wegensteiner, Rudolf ; Stauffer, Christian ; Cognato, Antony I.

Izvornik
Periodicum biologorum (0031-5362) 110 (2008), 4; 329-334

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Bark beetles; silver fir; morphology; mtDNA

Sažetak
The three palearctic species Pityokteines spinidens, P. curvidens and P. vorontzowi are main pests on Abies species and their impact on Abies-stands is increasing. As the three scolytid species are difficult to distinguish, this study aimed to find additional characters for identification. This is especially true regarding the females. Further, part of the mitochondrial COI gene was sequenced to obtain a first phylogenetic insight into their relationships and to develop barcoding markers for future use. All three bark beetle species were collected from logs in Litorić and Trakošćan, in order to quantify the number of strial and interstrial punctures. Insect DNA was extracted and PCR products were purified and directly sequenced with UEA10 on an ABI 3770 capillary sequencer, aligned using Clustal X v1.83. In addition to MP analysis, Bayesian analysis was performed by MRBAYES version 3.1.1 using the nucleotide substitution model by MRMODELTEST v2.1. The number of punctures in the first and second interstriae between the elytral base and the sutural tubercle proved to be a valuable tool for the differentiation of P. spinidens from P. curvidens and P. vorontzowi. This morphological feature was consistent with the number of punctures which varied for the first and the second interstriae in P. spinidens compared to P. curvidens and P. vorontzowi. The mitochondrial COI gene provided another mean in the discrimination of Pityokteines species, revealing that P. curvidens and P. vorontzowi are sister species.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Šumarstvo, Biotehnologija

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