Pregled bibliografske jedinice broj: 382430
Heterogeneity of DNA content in laryngeal squamous cell carcinoma in relation to histopathological variables
Heterogeneity of DNA content in laryngeal squamous cell carcinoma in relation to histopathological variables // Acta Oto-Laryngologica, 129 (2008), 7; 768-773 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 382430 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Heterogeneity of DNA content in laryngeal squamous cell carcinoma in relation to histopathological variables
Autori
Ladika-Davidović, Blaženka ; Ćorić, Marijana ; Bura Miljenko ; Brčić, Luka ; Kusić, Zvonko ; Seiwerth, Sven
Izvornik
Acta Oto-Laryngologica (0001-6489) 129
(2008), 7;
768-773
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
DNA content; heterogeneity; flow cytometry; laryngeal carcinoma; prognostic factors
Sažetak
Laryngeal carcinomas are characterized by considerable histological variation within the tumor. The possible effects of this morphological heterogeneity on the estimation of tumor DNA content were investigated. We analyzed 92 cases of archived, paraffin-embedded laryngeal carcinoma. For each tumor, three different regions, defined microscopically (center of tumor, transformation border, invasive border), were analyzed. DNA content was measured by flow cytometry. Three parameters were evaluated: DNA ploidy, DNA index, and proliferative index. DNA ploidy, DNA index, and proliferative index were higher in the tumor center than in the respective borders. Intratumoral differences in DNA ploidy were observed in 56 tumors (60.8%) ; DNA index heterogeneity was observed in 57.6% cases. Proliferative index varied greatly in morphologically different parts of the same carcinoma. DNA ploidy and DNA index of the transformation border correlated with tumor size and lymph node status. Proliferative index of the tumor center and of transformation border correlated with lymph node status, tumor size, and histological grade. Multivariate analysis showed age, lymph node status, proliferative index in transformation border, and DNA index in the center of laryngeal squamous cell carcinoma to be significant predictors for survival. By accessing distinctive areas of the tumor we were able to reduce the impact of intratumoral heterogeneity on prognostic factors.
Izvorni jezik
Engleski
Znanstvena područja
Kliničke medicinske znanosti
POVEZANOST RADA
Projekti:
053-0532264-2260 - Komparativna dijagnostika, morfometrija i analiza tumora ljudi i životinja (Grabarević, Željko, MZOS ) ( CroRIS)
108-0000000-0051 - Molekularna osnova raka štitnjače-kriteriji u terapiji diferenciranih karcinoma (Bura, Miljenko, MZOS ) ( CroRIS)
108-1083570-3643 - Kvantitativna analiza i prijenos slike u patologiji (Seiwerth, Sven, MZOS ) ( CroRIS)
108-0532264-0048 - Hepatocelularni tumori (Ćorić, Marijana, MZOS ) ( CroRIS)
134-1342428-2430 - Karcinogeneza u štitnjači i gušavost u Hrvatskoj (Kusić, Zvonko, MZOS ) ( CroRIS)
Ustanove:
Medicinski fakultet, Zagreb
Profili:
Blaženka Ladika Davidović
(autor)
Luka Brčić
(autor)
Sven Seiwerth
(autor)
Marijana Ćorić
(autor)
Miljenko Bura
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE