Naslov
Salviae officinalis L. as source of stilbene derivatives: Influence of the extraction mode

Autori
Generalić, Ivana ; Ljubenkov, Ivica ; Katalinić, Ana ; Katalinić, Višnja

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Proceedings of the Joint Central European Congress: 4th Central European Congress on Food and 6th Croatian Congress of food technologists, biotechnologist, and nutritionists (CEFood2008), 15-17 May 2008 Cavtat, Croatia ; Proceedings 2008, Vol. I ; ISBN 978-953-6207- 87-9 / Čurić, Duška - Zagreb : Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, 2008, 403-410

Skup
Joint Central European Congress: 4th Central European Congress on Food and 6th Croatian Congress of food technologists, biotechnologist, and nutritionists (CEFood2008), 15-17 May 2008 Cavtat, Croatia

Mjesto i datum
Cavtat, Hrvatska, 14.05.2008. - 17.05.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Salviae officinalis ; stilbenes ; HPLC ; extraction mode

Sažetak
Medicinal plants from the Mediterranean part of Croatia are traditionally used in our folk medicine due to their potential positive effect on human health. Numerous studies have already proved that some stilbene polyphenols, especially resveratrol (trans-3, 5, 4'-trihydroxystilbene), have good antioxidative, cardioprotective, antiinflammatory, and antitumour properties. The aim of this study was to research the potential of Salviae officinalis L., one of the most characteristic plants of the Dalmatian karst, as a source of resveratrol and its derivatives. The impact of maceration time (plant material-solvent contact time), temperature, stirring and ultrasonication on the efficiency of stilbene extraction was analyzed as well. Total phenols, flavonoids and nonflavonoids were analyzed by Folin-Ciocalteu method. The separation and quantification of stilbenes was done by HPLC-RP-PAD, using internal standard method. The results of the research strongly indicate that resveratrol or its hydroxy and methoxy derivatives are present in row plant material as glucosides. Free resveratrol monomers, if found, were present in very small amounts. Using conventional solvent extraction the best effect was obtained at 60 °C in duration of 60 minutes. Stirring didn’ t improve the effect of extraction. The increase in temperature from 60 to 75 °C resulted in an improved extraction of total phenols but not of resveratrol derivatives. While the ultrasonication-assisted extractions provided very good results, the conventional solvent extraction followed by the ultrasonication resulted in significant decrease of stilbene derivatives in plant extracts. According to the obtained results the leaves of Salviae officinalis contain resveratrol derivatives in amount enough high to be considered a valuable source of these biologically active polyphenols.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Prehrambena tehnologija

POVEZANOST RADA