Naslov
Demostration and quantification of sheep-associated malignant catarrhal fever agent in a heifer in Croatia

Autori
Turk, Nenad ; Freiss, Martina ; Grabarević, Željko ; Barbić, Ljubo ; Gudan, Andrea ; Milas, Zoran ; Starešina, Vilim

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract Book of the 2nd FEMS Congress of European Microbiologists / - Madrid : Federation of European Materials Societies (FEMS), 2006, 148-148

Skup
FEMS Congress of European Microbiologists (2 ; 2006)

Mjesto i datum
Madrid, Španjolska, 04.07.2006. - 08.07.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Ovine herpesvirus 2; malignant catarrhal fever; heifer

Sažetak
Ovine herpesvirus-2 (OvHV-2), a gammaherpesvirus (genus Rhadinovirus) causes the severe disease known as sheep-associated malignant catarrhal fever (SA-MCF) in certain ruminants, such as cow, deer, bison and water buffalo. Suspected cases of SA-MCF in cows without identification of the agent have already been reported in Croatia during past years. In June 2005, on a farm in north-west Croatia, where 17 Simmental diary cows and 2 heifers shared stables and meadows with sheep, a 13 month-old heifer showed symptoms reminiscent of SA-MCF, like anorexia, high fever, nasal discharge, and neurological symptoms, such as ataxia, tremor, convulsions and hyperesthesia. The animal died within 14 days. Gross necropsy findings were sharply demarcated erosions on the mucosal surfaces, including tongue, oral mucosa, esophagus, abomasums, jejunum, colon, caecum and urinary bladder. Histopathology revealed very severe perivascular and intramural arterial infiltrations with mononuclear cells, mostly lymphocytes. These lesions were seen in almost every organ, especially brain and lungs. Formaldehyde fixed samples from brain, cerebellum, spleen and lymph nodes were obtained and submitted to DNA extraction procedures. Fluorogenic real-time PCR (Polymerase chain reaction) amplification specific for OvHV-2 DNA was performed and OvHV-2 DNA was detected in brain, cerebellum, spleen as well as lymph nodes. This data indicates that the corresponding animal had been infected with OvHV-2, the agent of SA-MCF. For the first time, OvHV-2 was identified and quantified in one Croatian cow as the causative agent of SA-MCF.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA