Naslov
5-azacytidine seems not to disturb odontogenesis in rat embryonic mandible transplants to ectopic site

Autori
Turković, I ; Jurić-Lekić, Gordana ; Bulić-Jakuš, Floriana ; Lokosek, Vedran ; Radujković, Vedran ; Vlahović, Maja ; Katušić, Ana ; Šerman, Ljiljana ; Sinčić, Nino

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Aca Clinica Croatica 47 / - Zagreb, 2008, 18-18

Skup
19th Ljudevit Jurak International Symposium on Comparative Pathology

Mjesto i datum
Zagreb, Hrvatska, 06.06.2008. - 07.06.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
5-azacytidine; mandible; rat; in vivo transplantation; differentiation

Sažetak
In this study the differentiation of the rat embryonic mandibular tooth germs under the influence of the DNA demethylating drug 5-azacytidine (5azaC) was investigated. 13- and 14-days-old Fisher rat embryonic mandibles were microsurgically isolated under the dissecting microscope. Adult male rats of the same strain were anesthesized and mandibles were transferred by the braking pipette to the subcapsular kidney space. 5azaC (5mg/kg) was applied intraperitoneally during three days to the experimental group of animals while controls were treated by PBS. After two weeks, transplants were fixed for routine histology, embedded in parafin and sectioned. In both experimental and control group of animals, transplants were surviving well. Dental germs have developed to the bell stage of tooth development and, apparently, no differences were found in odontogenesis of the experimental and control group of transplants. Because it is known that 5azaC can cause changes in gene expression and disturb development, further studies are to be done to investigate whether odontogenesis in this system is really less susceptible to this DNA demethylating agent.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

Napomena
Nagrada na simpoziju za najbolji poster

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