Naslov
Glycosphingolipid expression in neural tissues of three rat strains

Autori
Čikeš Čulić, Vedrana ; Rešić, Jasminka ; Tomasović, Maja ; Zemunik, Tatijana ; Markotić, Anita

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Congress of the Croatian Society of Biochemistry and Molecular Biology with international participation : HDBMB 2008 : book of abstracts / Strelec, Ivica ; Glavaš-Obrovac, Ljubica - Osijek : Hrvatsko društvo za biokemiju i molekularnu biologiju (HDBMB), 2008, 78-78

ISBN
978-953-95551-2-0

Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology with international participation

Mjesto i datum
Osijek, Hrvatska, 17.09.2008. - 20.09.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
glycosphingolipid; neural tissues; rat strains

Sažetak
Many neurological disorders and injuries such as Parkinson's disease, multiple sclerosis, stroke, traumatic injury to CNS etc. have movement abnormalities as major symptom. Numerous animal models of neurological disease and injury exist, especially in rats, and most common used strains are Lewis, Wistar and Sprague-Dawley (SD). These three strains show different neurological behaviour. Glycosphingolipids (GSLs), both neutral and gangliosides are, according to their role, differently distributed in neural tissues and synaptic mechanisms. Therefore, comparison of GSL expression in neural tissues of three rat strains was performed. The aim was to identify GSL profile in brain, cerebellum and brain stem of each rat strain which could be responsible for different neurological behaviour. Three different male, age-matched rat strains were used: Lewis, Wistar and Sprague-Dawley. GSLs were isolated from animal tissues by standard procedure. Distribution of GSLs in brain, cerebellum and brain stem of each group of animals were analyzed by orcinol staining. GSLs were abundant in the brain of Lewis rats compared to lower expression in Wistar brain and traces of GSLs in SD brain. Cerebellum and brain stem of Wistar rats showed the highest GSL expression. Lewis cerebellum and brain stem showed lower expression of gangliosides and higher of neutral GSLs compared to the same Wistar tissues. SD cerebellum and brain stem showed traces of gangliosides and abundant expression of neutral GSLs. We define GSL profile in brain, cerebellum and brain stem of f Wistar, Lewis and SD rats which could be starting point for choice and defining neurochemical experiments with those strains.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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