Naslov
Expression of erbB-3 protein in colorectal adenocarcinoma: correlation with poor survival

Autori
Kapitanović, Sanja ; Radošević, Senka ; Slade, Neda ; Kapitanović, Milan ; Anđelinović, Šime ; Ferenčić, Željko ; Tavassoli, Mahvash ; Spaventi, Šime ; Pavelić, Krešimir ; Spaventi, Radan

Izvornik
Journal of cancer research and clinical oncology (0171-5216) 126 (2000), 4; 205-211

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
erbB-3 ; colorectal carcinoma ; survival ; prognosis

Sažetak
Background/Aims: The family of erbB receptors includes four transmembrane glycoproteins with tyrosine kinase activity. These receptors are widely expressed in normal tissues, but they also have been implicated in the development of several human adenocarcinomas. c-erbB-3/HER-3 has been detected to a greater or lesser extent in many tissues from the digestive, urinary, reproductive and respiratory tracts. The overexpression of c-erbB-3/HER-3 protein has also been shown in 53-88% of colorectal adenocarcinomas. In this study we investigated the expression of c-erbB-3/HER-3 gene product in colorectal tumor samples, and compared the results obtained with several clinicopathological parameters, including the survival of patients. Methods: Paraffin tissue sections were analyzed immunohistochemically using monoclonal antibody RTJ1 to human erbB-3 protein. Specificity of antibodies RTJ1 was confirmed by immunoprecipitation followed by Western Blotting analysis. Amplification of erbB-3 oncogene was tested by dot blot hybridization. Results. Adenocarcinomas of the colon were positive for erbB-3 protein in 78% of samples examined. Dot-blot analysis showed no amplification of erbB-3 gene in colon adenocarcinomas. Statistical analysis showed that patients with tumors that could not be stained for erbB-3 protein survived significantly longer (p<0.05) than patients with erbB-3 protein positive staining. A Cox proportional hazards model with stepwise variable selection identified age, sex and erbB-3 expression as important prognostic factors. Conclusion: These findings demonstrate that the erbB-3 protein expression could serve as an prognostic factor in colorectal malignancies.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA