Naslov
HORMONAL TREATEMENTS OF RECIPIENT DOES AND SEMILAPAROSCOPIC EMBRYOTRANSFER

Autori
Grizelj, Juraj ; Vince, Silvijo ; Baril, Gerard ; Lojkić, Martina ; Savic, Misha ; Getz, Iva ; Prvanović, Nikica ; Turmalaj, Luigj ; Đuričić, Dražen ; Samardžija, Marko ; Tariba, Bruna ; Mitak, Mario

Izvornik
Revista Romana de Medicina Veterinara (1220-3173) 18 (2008), 4; 172-175

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
goat ; recipient ; direct embryotransfer ; semilaparocopic

Sažetak
This article examines the procedure of recipient does hormonal preparation and the technique of semilaparoscopic embryotransfer. Furthermore, the direct transfer technique of thawed embryos was discussed, as well as the obstacles associated with those procedures which should significantly contribute to genetic improvement of the flocks. The hormonal preparation of recipient does was consisted of insertion of vaginal sponges during 11 days and application of eCG (equine chorionic gonadotrophin) and prostaglandins on day 9. 24h after sponges have been removed, detection of oestrus is performed. Seven days later semilaparoscopic direct transfer of thawed embryos is performed. Before the transfer, uterus, ovaries and the quality of corpora lutea were visually evaluated by laparoscope. There should be at least one functional corpus luteum (even better 2 of them), red in colour and 6-7 days old in order to use the goat as a recipient. Late ovulations as well as early luteolisis should be avoided. The transfer was accomplished by fixing and punctuation of the chosen horn near the uterotubal junction. It is important not to pass more than 1 minute from thawing till transfer of embryos. The direct transfer consist of thawing of the straw, after which the embryos are not passed through decreasing concentration of cryoprotector solutions, but are immediately transferred into the uterine horn. Such a direct transfer is possible because the both extreme parts of the straw were charged with sucrose.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA