Naslov
The use of the hypoosmotic swelling test and supravital staining in evaluation of sperm quality in boars

Autori
Samardžija, Marko ; Dobranić, Tomislav ; Krušlin, Suzana ; Cergolj, Marijan ; Karadjole, Martina ; Prvanović, Nikica ; Grizelj, Juraj

Izvornik
Veterinarski arhiv (0372-5480) 78 (2008), 4; 279-287

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
boar ; semen ; hypoosmotic test ; eosine/nigrosine staining

Sažetak
Semen samples from ten boars, Yorkshire and Pietrain breed, aged between two and six years were used. The sperm quality was evaluated using the following parameters: the motility (subjective), the structural supravital staining (eosine/nigrosine) and the functional integrity of sperm membrane (HOS test). The majority of HOS reactive or swelled up spermatozoa were gained in a hypoosmolar solution of 100 mOsm/kg by incubation of 30 minutes. Between middle values for HOS reactive (swollen up) spermatozoa in hypoosmolar solutions with different osmolarity, significant differences (P<0.05) were determined. Using a hypoosmotic solution with different osmolarity 100 mOsm/kg, significantly strong, positive correlations between HOS and motility (r = 0.98 ; P<0.05) were determined, between HOS test and supravital staining eosine/nigrosine (r = 0.99 ; P<0.05) and between motility and supravital staining eosine/nigrosine (r = 0.98 ; P<0.05). The results of our research have shown that the HOS test and supravital staining eosine/nigrosine could be used for routine evaluation of the functional and structural integrity of diluted boar sperm.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

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