Pregled bibliografske jedinice broj: 37429
Molecular structure of spinach chloroplast nucleoids
Molecular structure of spinach chloroplast nucleoids // Acta botanica Croatica, 58 (1999), 15-25 (podatak o recenziji nije dostupan, znanstveni)
CROSBI ID: 37429 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Molecular structure of spinach chloroplast nucleoids
(Molecular structure of spinach chloroplast nucleoid)
Autori
Fulgosi, Hrvoje ; Ljubešić, Nikola
Izvornik
Acta botanica Croatica (0365-0588) 58
(1999);
15-25
Ključne riječi
spinach; chloroplast nucleoids; cpDNA; proteins; microscopy
Sažetak
The ultrastructure of molecular composition of spinach chloroplast nucleoids were studied. Isolated nucleoids retained their thre-dimensionally folded stucture observed in situ. Fluorescence microscopy and staining with DAPI reaffirmed the mostly DNA-containing nature of these complexes. The presence of chloroplast DNA was confirmed by restriction fragment analysis. Electron microscopy revealed a highly condensed, chromatin-like, beaded structure. Naked DNA supercoils or loops were not observed. Integral nucleoid proteins were isolated and analysed on SDS-polyacrylamide gels. A distinct pattern of the nucleoid proteins, covering the entire range of molecular masses from 5 kDa to > 100 kDa revealed that the nucleoid fraction was highly depleted of major thylakoid and stroma constituents.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
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Časopis indeksira:
- Scopus
Uključenost u ostale bibliografske baze podataka::
- Biological Abstracts
